鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选  

Isolation and Screening of Antagonistic Bacteria Against Aeromonas hydrophila from Intestine of Anguilla anguilla

作　　者：张子华[1] 鄢庆枇[1,2] 邹文政[1,2] 邢颜丽[1,2] 郭松林[1,2] 纪荣兴[1,2] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2010年第3期165-171,共7页Journal of Jimei University：Natural Science

基　　金：国家863计划项目(2007AA09Z115);厦门市科技项目(3502Z20073019;3502Z20073020)

摘　　要：从鳗鲡肠道中分离到643株细菌,用点种法筛选出65株病原性嗜水气单胞菌的拮抗菌,研究拮抗效果最好的14株拮抗菌的抗菌谱和生长曲线及其与病原性嗜水气单胞菌的共凝集作用.研究结果显示:NAC琼脂培养基筛选的2株拮抗菌(Na 3-38和Nb 3-15)对7株指示菌均有较强的拮抗作用,Aa 3-22和Mb 3-7对7株指示菌中的5株有拮抗效果;除Na 3-38和Nb 3-15以外,其余12株的生长系数均大于嗜水气单胞菌;11株拮抗菌(Na 3-38、Nb 3-15、Ab 2-55、Ab 2-77、Ab 1-3、Aa 3-22、Aa 3-67、Mb 1-4、Mb 2-10、Mb 3-7和Mb 3-27)和嗜水气单胞菌的共凝集率大于10%.这表明:Aa 3-22和Mb 3-7在抗菌谱和生长曲线及其与病原性嗜水气单胞菌的共凝集作用等方面都适合作为益生菌.643 strains were isolated from healthy Anguilla anguilla intestinal tract,65 strains of antagonistic bacteria against Aeromonas hydrophila were identified by dot-inoculating method,and 14 of the 65 strains with strong antagonistic activity were chosen for antibacterial spectra,growth curves and co-agglutination assay.The results showed：2 isolated strains（Na3-38 and Nb3-15） ,had a wider range of antibacterial spectra than others,5 of 7 indicating bacteria were obviously inhibited by isolated strains of Aa3-22 and Mb3-7;12 isolated strains had more advantage in R than A.hydrophila;the co-agglutination ratio was more than 10 % between 11 isolated strains and A.hydrophila.The results indicated that the isolated strains of Aa3-22 and Mb3-7 could be used to develop probiotics based on their antibacterial spectrum,growth curve and the co-agglutination with A.hydrophila.

关 键 词：欧洲鳗鲡 嗜水气单胞菌 拮抗菌 抗菌谱 生长曲线 共凝集 

分 类 号：S941[农业科学—水产养殖]