日本鳗鲡不同组织器官的抗菌活性比较  

Antibacterial Activities in Different Tissues or Organs of Japanese Eel,Anguilla japonica

作　　者：徐同玲[1] 关瑞章[1,2] 梁英[1,2] 黄文树[1,2] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2010年第3期172-178,共7页Journal of Jimei University：Natural Science

基　　金：农业部公益性行业(农业)科研专项(nyhyzx07-043);福建省科技厅高校专项(2007F5079);集美大学博士启动基金项目(ZQ2006024)

摘　　要：用体积分数为10%的醋酸提取日本鳗鲡肝脏、鳃、脾脏、胆汁、肾脏和血清中的抗菌活性物质,检测其对养殖鳗鲡常见的4种致病菌,即肠杆菌Enterobacter sp.(B01)、迟缓爱德华氏菌Edwardsiellatarda(B09)、气单胞菌属Aeromona sp.(B18)和嗜水气单胞菌Aeromona hydrophila(B27)的抑杀作用.结果显示:1)肝脏、脾脏、胆汁和鳃的样品对4株试验菌株均有明显的抑菌作用,肾脏的对B18、B27和B013株致病菌有抑菌作用,血清的仅对B18有抑菌作用.2)不同样品对同一种致病菌的抗菌活性差异显著(P<0.05),按平均抑菌圈半径计算,其抗菌活性大体呈现为:肝脏>鳃>脾脏>胆汁>肾脏>血清;同一样品对不同细菌的抑杀作用差异显著(P<0.05).采用反相高效液相色谱法分析上述各组织/器官的醋酸提取物中的活性组分,结果显示:肝脏、脾脏、胆汁和鳃样品中的活性组分(以杀伤指数大于50%计)主要集中于乙腈洗脱质量分数为19.4%～35.4%时,肾脏和血清中没有明显的抗菌作用(杀伤指数小于50%).Compared and analysed of the antibacterial activities in different tissues and organs,including liver,gill,spleen,bile,kidney and serum of A.japonica.This study obtained acid extractions of the tissues and organs from Japanese eels.Using agar diffusion method,the antibacterial activity of those extractions against four common pathogenic bacteria（Enterobacter spp.B01,Edwardsiella tarda B09,Aeromonas spp.B18 and A.hydrophila B27） from the cultivated eels was detected.The results showed that antibacterial activities of liver,gill,spleen,bile were obvious.The extractions of kidney exhibited antibacterial activity against B18,B27 and B01.The extractions of serum exhibited antibacterial activity against B18.The antibacterial activities of extractions from different tissues and organs were different to the same bacterium.The antibacterial activity of liver was the highest to all experimented bacteria.The antibacterial activity of extractions from the same tissues or organs varied to different bacteria.The acid extractions were further purified by the method of reverse-phase liquid chromatography on Source 5R RPC-ST-4.6/150 column（Amersham Bioscience,Sweden） .The results showed that the killing indexes of the extractions from liver,kidney,gill and bile were higher than 50 % when eluted by the concentration of aceonitrile ranging from 19.4 % to 35.4 %,while the killing index of extractions from serum and kidney were lower than 50 % when eluted by the concentration of aceonitrile ranging from 0 % to 80 %.

关 键 词：日本鳗鲡 抗菌活性 反相高效液相色谱 

分 类 号：S941[农业科学—水产养殖]