HPLC法测定痛经贴膏中芍药苷的含量  

HPLC Determination of Paeoniflorin in Tongjing Plasters

作　　者：孙峰[1] 李贤春[1] 姚宏涛[2] 

机构地区：[1]沈阳东陵药业股份有限公司,辽宁沈阳110161 [2]沈阳禾晶医药科技有限公司,辽宁沈阳110015

出　　处：《辽宁中医杂志》2010年第5期898-899,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:建立痛经贴膏中白芍苷的含量。方法:色谱柱:YWG-C18柱(250mm×4.6mm,5μm,由迪马公司提供),流动相为甲醇-水(35∶65),检测波长230nm。结果:芍药苷在2.5～40μg的浓度范围内峰线性关系良好(r=0.9999),平均回收率在96.13%(n=6)。结论:该方法准确可靠,简单快速,可用于测定痛经贴膏中白芍苷的含量。Objective：To establish a HPLC method for determination of paeoniflorin in Tongjing Plasters.Methods：HPLC conditions were as follows：a YWG-C18 column（250mm×4.6mm,5μm）,a mobile phase：methanol：water（35∶65）,a detection wavelength of 230nm.Results：The linear range for paeoniflorin was 2.5~40μg（r=0.9999）,the average recovery was 96.13%（n=6）.Conclusion：A simple,rapid,and accurate method for determination of paeoniflorin in Tongjing Plasters is established.

关 键 词：痛经贴膏 芍药苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]