不同含油量木鳖子霜毒效关系研究  

The Study of Relation of Toxicity and Pharmacodynamic Action With Cochinchina Momordica Seed Crem With Eifferent Oil Content

作　　者：孙付军[1] 路俊仙[1] 崔璐[2] 陈慧慧[2] 王春芳[2] 林慧彬[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250014

出　　处：《辽宁中医杂志》2010年第5期946-948,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金："十一五"国家支撑计划课题资助项目(2006BAI09B00605)

摘　　要：目的:分别观察不同含油量木鳖子霜急性毒性与药效学作用,探讨毒性与药效学的关系,为制霜工艺提供毒理和药效学依据。方法:按照急性毒性试验LD50测定方法,灌胃给予10%、20%、30%含油量木鳖子霜和木鳖子原药材的水混悬液,观察其急性毒性,Bliss法计算各自的LD50和95%可信度区间。采用小鼠耳肿胀法和小鼠热板法观察不同含油量木鳖子霜的抗炎、镇痛作用,观察小鼠体重、脏器指数、耳肿胀率、痛阈值等指标。结果:10%、20%、30%含油量木鳖子霜和原药材的LD50分别为3.923、5.6393、5.9594、6.1610g/kg。不同含油量木鳖子霜对小鼠体重、脏器指数、耳肿胀率、痛阈值等指标有不同程度影响,小鼠一般状况较空白对照组差,体重普遍低于空白对照组;对小鼠主要免疫器官具有不同程度的抑制作用;具有明确的抗炎和镇痛作用,但对镇痛试验的舔足次数无显著性影响。上述所有指标均未呈现明显的线性量效关系,随含油量的增加,均呈现出先增强后减弱的变化趋势,其中10%～20%含油量时其抗炎、镇痛作用最好,且对体重和免疫器官的影响最小。结论:木鳖子毒性随着含油量的增大呈现降低趋势,木鳖子在20%含油量时抗炎、镇痛等药效学作用最为明显,对免疫器官的抑制作用最小,对小鼠一般状况和体重影响最小。Objective：Observing acute toxicity and pharmacodynamic action of cochinchina momordica seed crem with different oil content respectively,and discussing their relation prived basis for frost-like powder making artwork in toxicology and pharmacodynamics.Methods：According to LD50 assay method in the acute toxicity test,lavaged aqueous suspension with 10%,20%,30% index of oil momordica cochinchinensis sub-frost and the momordica cochinchinensis original raw material observed its acute toxicity,and calculated respective LD50 and 95% confidence level sector in the Bliss law.Adopt ear edema and hot plate method in mice to observe anti-inflammatory and analgesic effect of different index of oil momordica cochinchinensis.Results：the LD50 of 10%,20%,30% index of oil momordica cochinchinensis sub-frost and the momordica cochinchinensis original raw material is 3.923g/kg,5.6393g/kg,5.9594g/kg,6.161g/kg.Cochinchina momordica seed crem with different oil content play a part of different degree in mouse weight、organ index、ear swelling rate、pain threshold and so on.The mouse general condition is worse than the mouse in blank and the body weight is lower,too;Having the varying degree inhibitory action to the mouse main immune organ;Having an expect anti-inflammatory and analgesic effect,but it has no significant impact for Licking test of analgesia sufficient number.All of these indicatrix have not shown clear signs of a linear dose-effect relationship,with the increase in oil content,were later shown to enhance the trend of weakening,of which 10%~20% oil content in its anti-inflammatory and analgesic effect of the best,and of body weight and minimize the impact of immune organs.Conclusion：sion：acute toxicity of cochinchina momordica seed crem Decreased trend with improved of oil content,cochinchina momordica seed crem oil content of 20% when the anti-inflammatory,analgesic pharmacodynamics,such as the role of the most obvious inhibitory effect on the immune organs of the smallest,in mice the general situation and

关 键 词：木鳖子 急性毒性 药效学 小鼠 

分 类 号：R285.5[医药卫生—中药学]