大鼠膈神经异化支配迷走神经的Y管实验研究  

Experimental study on phrenic-vagus heterogenic reinnervation in a rat silicone Y chamber model

作　　者：叶晓生[1] 张世民[1] 杜守超[1] 

机构地区：[1]同济大学附属同济医院骨科,上海200065

出　　处：《同济大学学报（医学版）》2010年第2期27-31,37,共6页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金资助项目(30672139)

摘　　要：目的探讨大鼠膈神经经Y形管长入迷走神经的异化再生特征及神经生长因子(nerve growth factor,NGF)对其再生的影响。方法 SD大鼠20只,颈部建立膈神经-迷走神经"Y形再生室"异化神经模型(硅胶Y管近端接膈神经近端,远端接迷走神经和膈神经远端),随机分成2组,A组(对照组)10只,术后腹腔注射生理盐水,B组(NGF组)10只,术后腹腔注射NGF,连续2周。术后12周电刺激检测大鼠的心脏功能,对再生神经进行组织学观察。结果电刺激再生异化神经后,B组大鼠动脉血压及心率的下降幅度均大于A组,差异有统计学意义(P<0.05);B组再生异化神经和膈神经有髓纤维数目、通过率、髓鞘厚度和轴突直径均大于A组,差异有统计学意义(P<0.05);同组两侧比较,再生有髓神经数目和通过率为异化神经侧多,髓鞘厚度和轴突直径为膈神经侧大,差异有统计学意义(P<0.05)。结论异化神经具有成熟髓鞘结构及靶器官支配功能,在性质上属于躯体运动神经;NGF能够促进异化神经生长并提高其支配效能;再生神经无明显解剖部位趋化性,长入远端神经干粗大侧(迷走神经)有数量优势,长入躯体运动神经侧(膈神经)有质量优势。Objective To study the regeneration characteristics of Phrenic-Vagus heterogenic reinnervation and its influences by nerve growth factor（NGF） injected intraperitoneally in a silicone Y chamber model in rats. Methods Twenty SD rats were used to establish the model of phrenic- vagus heterogenic reinnervation in a silicone Y chamber and were randomly divided into two groups. Phrenic and vagus nerves were transacted with the proximal stump of phrenic nerve being inserted into the proximal stump of the silicone Y tube and the distal two stumps being inserted by the distal stumps of vagus nerve and phrenic nerve. After intraperitoneally injected with NGF, while the other saline. Twelve weeks later,the cardiac function operation ,NGF group（B, 10 in number） was group（A, 10 in number） was treated with control was examined by electrical stimulation of the regenerated heterogenic nerve. Light and electron microscopic analysis was applied to examine the number,axonal transition ratios,the thickness of myelin sheath and axonal diameter of regenerated nerves. Results Under electrical never stimulation, significantly decreased blood pressure and heart rate were found in B group as compared to those in A group. Morphological examinations revealed that the heterogenic nerve fibers were able to regenerate into vagus nerve with biological effect as demonstratedly the structure of matured myelin sheath and the axons from heterogenic nerve. The number,axonal transition ratios,myelin sheath thickness and axonal diameter of the regenerated myelinated nerve in non-NGF treated animals were significantly higher than those in NGF-treated animals. Furthermore,the number and transition ratios of the regenerated myelinated nerve was significantly higher in the heterogenic side than that in the unheterogenic phrenic side; but the thickness of myelin sheath and axonal diameter was higher in the unheterogenic phrenic side. Conclusion Heterogenic nerve is primarily a somatic motor nerve; NGF can promote the axons of heterogenic nerve

关 键 词：膈神经 迷走神经 异化神经再支配 神经再生趋化性 神经生长因子 

分 类 号：R622.3[医药卫生—整形外科]