乙型肝炎病毒抗原诱导T细胞受体Vβ基因亚家族特异性扩增与多样性  

Hepatitis B virus antigen-induced specific TCR Vβ gene subfamily amplifications and their diversity

作　　者：黄树林[1,2] 曹茂开[1,2] 罗利琼[1,2] 李依娜 李菁华[1,2] 

机构地区：[1]广东药学院分子生物学研究室 [2]西安交通大学生物工程系

出　　处：《中华实验和临床病毒学杂志》1998年第4期318-321,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金;广东五个一工程基金

摘　　要：目的对乙型肝炎疫苗注射前后及ＨｅｐＧ２２２１５上清ＨＢｓＡｇ、ＨＢｅＡｇ感染淋巴细胞，Ｔ细胞受体（ＴＣＲ）Ｖβ基因亚家族表达特征与多样性，为研究识别机制提供依据。方法采用ＲＴ－ＰＣＲ－Ｓｏｕｔｈｅｒｎ印迹扫描。结果乙肝疫苗注射后Ｖβ６、１４增高，ＨｅｐＧ２２２１５上清感染后Ｖβ６、１５增高。结论Ｖβｂ为两种抗原共同识别亚家族。Ｖβ１４。The human immune system confronts various antigens, then the recognitive structures of T lymphocytes must be diverse. In this study, we analyzed the TCR Vβ 1-20 gene repertoire by reverse transcription-polymerase chain reaction (RT-PCR) in peripheral blood lymphocytes (PBLs) from individuals vaccinated with recombinant hepatitis B vaccine and PBLs from healthy donors stimulated by supernatants of the HepG2 2215 cell line transfected by HBV-DNA. The specific amplifications of Vβ 6、14, Vβ 6、15 were observed in PBLs after vaccination and stimulation by HepG2 2215 cell line respectively. The results suggest that the Vβ segments may be the specific recognitive units for these antigens and involved in restriction and cytotoxicity.

关 键 词：乙型肝炎病毒 T细胞受体 基因表达 

分 类 号：R373.21[医药卫生—病原生物学]