广东地区肝炎患者血清中庚型肝炎病毒RNA的检测  

An analysis of hepatitis G virus infection status in various cases of hepatitis in Guangdong area

作　　者：姚春斓[1] 周元平[1] 谢冬英[1] 崇雨田[1] 李刚[1] 姚集鲁[1] 

机构地区：[1]中山医科大学附属第三医院传染病科

出　　处：《中华实验和临床病毒学杂志》1998年第4期330-332,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解庚型肝炎病毒（ＨＧＶ）感染在不同临床类型肝炎患者中的存在状况。方法用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术对７２１例肝炎患者血清进行了ＨＧＶＲＮＡ检测。结果发现８０例ＨＧＶＲＮＡ阳性，以非甲、乙、丙、丁或戊型肝炎中阳性率为高；慢性丙型肝炎（ＨＣＶ）和慢性乙型混合丙型肝炎（ＨＢＶ＋ＨＣＶ）次之；在慢性重型肝炎和肝硬化时较低。To understand hepatitis G virus(HGV)infection status in different clinical types of hepatitis,721cases with hepatitis were analyzed in this article.PT-PCR technique was applied to amplify HGVgene,and heat denaturation method was used to extract HCV-RNA from sera.After turning to cDNA by reverse transcription(PT),a nested amplification was conducted and a 238 bp specific fragment was obtained .In this way,serum specimens of 721 cases with hepatitis,were tested including 587 hospitalized cases.80 cases were found to be HGV RNA positive, of which positive rate of patients with non A E hepatitis was high,HCV and HBV+HCV came the second,chronic severe hepatitis and liver cirrhosis (LC)was the lower.Results of research showed that HGV infection is prevalent to a certain degree among patients with viral hepatitis in Guangdong area.

关 键 词：庚型肝炎病毒 聚合酶链反应 肝炎 检测 

分 类 号：R373.21[医药卫生—病原生物学]