复方SZ滴眼液和阿魏酸钠对紫外线损伤的人晶状体上皮细胞抗氧化酶及其基因表达的影响  

The Effect of Co SZ Eye Drop and Sodium Ferulate on Anti-oxidase and Its Gene Expression in Human Lens Epithelial Cell Injured by Ultraviolet

作　　者：黄秀榕[1] 祁明信[2] 严京[2] 

机构地区：[1]福建中医药大学病理生理研究中心,福建福州350003 [2]福建中医药大学附属第二人民医院眼科,福建福州350003

出　　处：《中华中医药学刊》2010年第5期904-907,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：福建省中医药重点项目(Wzzb0603)

摘　　要：目的:研究复方SZ滴眼液(Compound shui zhi eye drop,SZ)和阿魏酸钠(Sodium ferulate,SF)对紫外线(Ultraviolet,UV)损伤的人晶状体上皮细胞抗氧化酶及其基因表达的影响。方法:采用UV照射体外培养的人晶状体上皮细胞(Human lens epithelial cell,HLEC),同时用SZ、SF和吡喏克辛钠滴眼液(Pirenoxine sodium,PS)与HLEC共同孵育,并检测HLEC中超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathi-one peroxidase,GSH-px)和过氧化氢酶(Catalase,CAT)的活性和SODmRNA、GSH-pxmRNA和CATmRNA的基因表达。结果:UV组的HLEC抗氧化酶活性和基因表达均比对照组显著降低(P<0.01)。SZ、SF和PS组的SOD、GSH-px和CAT的活性显著高于UV组(P<0.01,P<0.05);SODmRNA、GSH-pxmRNA和CATmRNA的表达也显著高于UV组(P<0.01)。结论:SZ、SF和PS均能防护UV照射引起的HLEC氧化损伤,SZ和SF的作用优于PS。Objective：To investigate the effect of compound shui zhi eye drop （SZ） and sodium ferulate （SF）on anti-oxidase and its gene expression in human lens epithelial cell（HLEC） injured by ultraviolet（UV）.Methods：HLEC were irradiated by UV.The HLEC were incubated with SZ,SF and pirenoxine sodium（PS）.The activities of superoxide dismutase （SOD）.glutathione peroxidase （GSH-px ） and catalase （CAT） were measured and the gene expression of SODmRNA,GSH-pxmRNA and CATmRNA were detected.Results：The activities of anti-oxidase and its gene expression of HLEC in UV group decreased significantly compared with control group （P〈0.01）.The activities of SOD,GSH-px and CAT in SZ group,SF group and PS group were higher than that in UV group significantly （P〈0.01,P〈0.05）.The gene expression of SODmRNA、GSH-pxmRNA and CATmRNA in SZ group,SF group and PS group were higher than that in UV group remarkably （P〈0.01）.Conclusion：SZ,SF ang PS can prevent and protect oxidative damages in HLEC injured by UV;The effects of SZ and SF are better than PS.

关 键 词：白内障 人晶状体上皮细胞 紫外线 氧化损伤 基因表达 复方SZ滴眼液 阿魏酸钠 

分 类 号：R285.5[医药卫生—中药学]