对萼猕猴桃叶中黄酮类成分的含量分析  

Quantitative Analysis of Flavonoids in Leaves of Actinidia valvata

作　　者：吴迎春[1,2] 辛海量[2] 盛佳钰[2] 张亚妮[2] 凌昌全[2] 

机构地区：[1]上海中医药大学中药化学教研室,上海201203 [2]第二军医大学长海医院,上海200433

出　　处：《中华中医药学刊》2010年第5期954-958,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30572360);上海市科委中药现代化专项基金资助项目(04DZ19808)

摘　　要：目的:分析对萼猕猴桃叶中总黄酮以及黄酮类成分山柰酚-3-O-β-D-吡喃半乳糖苷(ry-1)、对萼猕猴桃苷E(ry-2)、对萼猕猴桃苷F(ry-3)的含量,为对萼猕猴桃叶品质评价和质量控制提供依据。方法:采用芦丁比色法对对萼猕猴桃叶中总黄酮进行含量分析;建立HPLC法对对萼猕猴桃叶中黄酮类成分ry-1、ry-2、ry-3进行含量分析。色谱柱:Shim-packCLC-ODS色谱柱(4.6mm×25mm,5μm);保护柱:DIKMAEasy-GuardC18(10mm×4.6mm),流动相:乙腈-0.5%醋酸水溶液梯度洗脱;柱温:26℃;检测波长:360nm;流速:1.00mL/min。结果:总黄酮的回归方程为A=8.26×10-2+1.90×10-3,r=0.9995,线性范围:0.01～0.09mg/mL;ry-1回归方程为A=6.84×10-5C+6.53×10-2,r=0.9999,线性范围:0.792～23.760μg/mL;ry-2回归方程为A=8.58×10-5C+0.124,r=0.9999,线性范围:2.570～77.100μg/mL;ry-3回归方程为A=7.89×10-5C+9.54×10-2,r=0.9999,线性范围:2.168～65.040μg/mL。结论:该方法前处理简便,分析准确、快速,可以作为对萼猕猴桃叶中总黄酮以及ry-1、ry-2、ry-3的定量分析方法,也为对萼猕猴桃叶的质量控制和品质评价提供了重要的依据。Objective：To determine content of total flavonoid and in leaves of Actinidia valvata,which may provide experimental evidence for its qualitative control and evaluation.Methods：Rutoside-UV method was used for the determination of total flavonoid and HPLC method was developed for the determination of kaempferol-3-O-β-D-galactopyranoside（ry-1）,actinoside E（ry-2） and actinoside F（ry-3） in leaves of Actinidia valvata.The HPLC method was developed with the conditions as following：Shim-pack CLC-ODS column（4.6mm×25mm,5μm）,DIKMA Easyguard C18 （10mm×4.6mm）,gradient elution was employed with the mobile phase acetonitrile-0.5% acetic acid solution at flow rate of 1.00mL/min;Column temperature：26 ℃;Detection wavelength：360 nm.Results：The standard curves of total flavonoid,ry-1,ry-2 and ry-3 were A=8.26×10^-2C＋ 1.90×10^-3 （r=0.9995）,A=6.84×10^-5C ＋6.53×10^-2 （r=0.9999）,A=8.58×10^-5C＋0.124（r=0.9999） and A=7.89×10^-5C ＋9.54×10^-2 （r=0.9999）,respectively.The standard curves of total ry-1,ry-2 and ry-3 were linear in the range of 0.01～0.09 mg/mL,0.792～23.760 μg/mL,2.570～77.100μg/mL and 2.168～65.040μg/mL,respectively.Conclusion：The method is feasible for quantitative analysis of total flavonoid,ry-1,ry-2 and ry-3 in leaves of Actinidia valvata,and also provided meaningful evidence for its qualitative control and evaluation.

关 键 词：对萼猕猴桃叶 黄酮 含量测定 HPLC 

分 类 号：R284.1[医药卫生—中药学]