^(32)P粒子植入对正常肝组织损伤及体内代谢的实验研究  

The metabolism of ^(32)P-CP-PLLA seed implanted in the liver and its damage to the normal liver tissue:a study in the experimental dogs

作　　者：谭中宝[1] 刘璐[2] 郭金和[1] 朱光宇[1] 王福安[1] 聂琦[2] 高海林[2] 滕皋军[1] 

机构地区：[1]南京东南大学附属中大医院介入科,21009 [2]东南大学临床医学院核医学技术研究所

出　　处：《介入放射学杂志》2010年第4期309-314,共6页Journal of Interventional Radiology

基　　金：国家高技术研究发展计划(863计划)(2007AA02Z471)

摘　　要：目的观察32P-磷酸铬-聚L-乳酸(32P-CP-PLLA)粒子对正常犬肝组织损伤效应,及其体内代谢和缓释效应。方法Beagle犬12只,随机分为4个剂量组,A组185 MBq、B组370 MBq、C组740MBq,D组为空白对照假手术组。采用开腹将32P-CP-PLLA粒子植入犬肝脏内,3个月后处死。术前及处死前均行CT扫描,术前及术后1、2、4、8、12周进行血象及血清生化检查,B、C两组各选1只于代谢笼内饲养,术后1个月内每24 h测量粪便及尿液放射性计数率值(cpm),术后1、2、4、8、12周每组选1只犬行肝穿刺及SPECT韧致辐射显像,处死时行病理学检查。结果所有实验犬血象及血生化各项指标未见明显异常;术后30 d的排泄物放射性检查表明粒子在体内缓慢释放;尿、粪排出的放射性计数累积百分率分别为6.34%和11.64%;SPECT显像示无粒子移位现象;3个月后解剖发现粒子体积变小、质地松脆,CT、大体标本和病理学检查结果均发现随着剂量增大,肝脏内粒子植入部位毁损灶增大。32P-CP-PLLA粒子造成的局部肝损伤呈一球形灶:纤维化组织包绕坏死灶,外周可见一圈水肿带。结论32P-CP-PLLA粒子植入犬肝脏后对肝组织内的损伤是局限的,不会造成全身的不良反应,在体内无脏器迁移,并呈现缓慢释放,显示出良好体内稳定性、靶向定位性和安全性。Objective To investigate the effects of intratumoral implantation of ^32P -CP-PLLA seeds on the normal canine liver tissue and to explore the metabolism of ^32P-CP-PLLA seeds implanted in the liver of experimental dogs. Methods Twelve beagles were enrolled in this study. The dogs were randomly and equally divided into four groups： group A （185 MBq）, group B （370 MBq）, group C （740 MBq） and group D （0 MBq）. By using laparotomy procedure 32P-CP-PLLA seeds were implanted into dog＇s liver. CT scan was performed before operation as well as before the dog was sacrificed. All dogs were sacrificed three months after the implantation. Before the procedure and 1, 2, 4, 8 and 12 weeks after the procedure the blood tests and serum biochemical tests were conducted. One dog from group B and group C was selected respectively and was fed in a metabolic cage. Within one month after the procedure the cpm in feces and in urine was determined every 24 hours. One dog was picked out from each of the three groups and was punctured to get its liver tissue for pathologic exam each time at 1, 2, 4, 8 and 12 weeks after the implantation, and SPECT imaging was also performed at the same time. Pathologic study, both macroscopic and microscopic （including optical and electronic microscopy） was made to observe the liver damage after the dog was sacrificed. The statistical analysis was processed by using SPSS 13.0 software and the measuring data were expressed with mean ± standard deviation （x ± s）. Results Two months after the procedure, serological examination found that the serum alkaline phosphatase （BKP） in both group B and group C was significantly higher than that in other groups, the difference was statistically significant （P 0.05）, and the BKP levels returned to normal in three months. The postoperative 30-day inspection of the urine showed that the radioactive particles slowly released into the body and eliminated from the body with the urine and feces, mainly through the renal excretion. The 30-

关 键 词：32P-CP-PLLA粒子 内放射治疗 间质 靶向定位 安全性 

分 类 号：R817[医药卫生—影像医学与核医学]