大鼠颅脑爆炸伤后脑皮质自噬变化的实验研究  

Experimental research of autophagic changes in the cerebral cortex after brain explosive injury in rats

作　　者：汤宏[1] 刘家传[1] 张永明[1] 杨艳艳[1] 孙文江[1] 张良潮[2] 李兵仓[2] 

机构地区：[1]安徽医科大学附属解放军105医院神经外科,合肥230031 [2]第三军医大学大坪医院野战外科研究所六室,重庆400042

出　　处：《中国微侵袭神经外科杂志》2010年第4期179-181,共3页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：南京军区十一五重点课题(编号:06Z19)

摘　　要：目的研究大鼠颅脑爆炸伤后脑皮质中自噬相关基因Beclin-1的表达变化情况。方法SD大鼠30只,随机分为对照组(n=6)和实验组(n=24)。实验组采用600mgTNT电雷管在大鼠头部正上方12cm处爆炸,以建立颅脑爆炸伤模型;对照组不行爆炸致伤。伤后6h、24h、3d、7d分别采用RT-PCR和Western blot方法检测脑皮质中Beclin-1的表达情况。结果对照组Beclin-1表达水平较低。实验组脑皮质中Beclin-1在伤后6h表达上升,伤后3d达高峰,伤后7d表达有所下降;与对照组比较,差异具有统计学意义(P<0.05)。结论大鼠颅脑爆炸伤后,脑皮质中Beclin-1表达呈波峰状,提示自噬现象在颅脑爆炸伤后增强,且可能参与颅脑爆炸伤神经元损伤后的应激反应。Objective To study the changes of autophagy-related gene Beclin-1 in the cerebral cortex after brain explosive injury in rats.Methods Thirty SD rats were divided into control group （n=6） and experimental group （n=24） at random.The rats were injured by 600 mg TNT electric detonators,which exploded at 12 cm distance over the rat head,as explosive injury model in the experimental group,and no explosive injury was performed in the control group.The changes of Beclin-1 expression in the cerebral cortex were detected by RT-PCR and Western blotting at 6,24 hours,and 3,7 days after injury.Results In contrast with low expression in the control groups,the expression of Beclin-1 in the experimental group increased at 6 hours after injury and reached peak at 3 days,and then decreased at 7 days.The changes were significantly different between control and experimental groups （P 0.05）.Conclusion The expression of Beclin-1 in the cerebral cortex presents ridge pattern after explosive injury,suggesting that autophagy is activated and might have responses to the stress of neuron injury after explosive injury.

关 键 词：颅脑损伤 自噬 大脑皮质 大鼠 

分 类 号：R651.15[医药卫生—外科学]