人羊膜细胞在Ⅰ型胶原基质中的三维培养研究  

Human Preterm Amnion Cells Cultured in Three-dimensional Collagen Ⅰ Matrix

作　　者：刘芳[1] 漆洪波[2] 

机构地区：[1]重庆市妇产科医院,重庆400016 [2]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《生物医学工程学杂志》2010年第2期384-388,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30772336)

摘　　要：将人羊膜间质细胞种入Ⅰ型胶原三维基质中,羊膜上皮细胞接种在基质的表面,构建人羊膜细胞/Ⅰ型胶原基质复合物以模拟人体羊膜组织结构,为其用于修复胎膜破口的研究做准备。采用罗丹明-鬼笔环肽荧光染色剂特异性标记胞浆中的微丝(F-actin)结构,观察细胞在三维基质中的细胞形态;扫描电镜和透射电镜法观察羊膜上皮细胞和间质细胞在三维培养中的超微结构。结果显示人羊膜上皮细胞和间质细胞在Ⅰ型胶原基质的复合培养中生长良好,显示出与体内羊膜细胞的相似性。以上结果提示,Ⅰ型胶原蛋白有望应用在羊膜细胞组织工程,三维细胞基质培养系统有望为胎膜早破提供新的研究方法和治疗手段。For the purpose of fetal membrane healing,amnion mesenchymal cells were embedded in three-dimensional（3D） collagenⅠ,and amnion epithelial cells were placed on top of it to build the amniotic cells/collagenⅠmatrix complex to simulate the architecture of native amnion.Morphology of cell cultured on 3D collagen I was visualized by F-actin filaments incubated with rhodamine-labelled phalloidin.Cell ultrastructure of amnion epithelial cells and amnion mesenchymal cells was visualized by scanning electron microscope（SEM） and transmission electron microscope（TEM）.Results show amnion epithelial and mesenchymal cells cultured in collagen I matrix assume cell morphologies similar to those observed in vivo.Conclusion： CollagenⅠ might be useful in amnion cell tissue engineering, Three-dimensional cell-matrix system might be useful in the study of preterm premature rupture of the membrane（PPROM） and be a therapeutic strategy for PPROM.

关 键 词：人羊膜细胞 Ⅰ型胶原蛋白 三维培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]