Foxp3在成神经细胞瘤细胞株SK-N-SH中的表达及其对化疗的敏感性  

Foxp3 expression in neuroblastoma cell line SK-N-SH and its sensitivity to chemotherapy

作　　者：孙婧[1] 李良[1] 肖燕[1] 唐锁勤[1] 

机构地区：[1]解放军总医院第一附属医院儿科,北京100037

出　　处：《中国肿瘤生物治疗杂志》2010年第2期179-182,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家高技术研究发展计划(863计划)资助项目(No.2007AA021004)~~

摘　　要：目的:研究成神经细胞瘤细胞株SK-N-SH中Foxp3的表达及其对化疗药物环磷酰胺(cyclophosvnamide,CTX)和吡柔比星(pirarubicin,THP)的敏感性。方法:体外培养SK-N-SH细胞,流式细胞术检测Foxp3在SK-N-SH细胞中的表达。MTT法检测化疗药物CTX、THP对SK-N-SH细胞的敏感剂量;流式细胞术及real-time PCR检测CTX、THP对SK-N-SH细胞中Foxp3表达的影响。结果:流式细胞术检测结果显示,SK-N-SH细胞高表达Foxp3分子。CTX作用于SK-N-SH细胞的敏感剂量为6mmol/L,THP作用于SK-N-SH细胞的敏感剂量为80ng/ml。6mmol/L CTX或80ng/ml THP以及两者的联合不能抑制SK-N-SH细胞中Foxp3的表达(P>0.05);real-time PCR结果也证实,CTX或THP以及两者的联合不能抑制SK-N-SH细胞中Foxp3 mRNA的表达。结论:成神经细胞瘤细胞株SK-N-SH高表达Foxp3蛋白,但其表达对化疗药物CTX和THP不敏感。Objective： To investigate Foxp3 expression in neuroblastoma cell line SK-N-SH and its chemosensitivity to cyclophosvnamide （CTX） and pirarubicin （THP）. Methods： SK-N-SH cells were cultured in vitro, and Foxp3 expression in SK-N-SH cells was examined by flow cytometry （FCM）. The sensitive dosages of CTX and THP on SK-N-SH cells were determined by MTr assay. The effects of CTX or THP on Foxp3 expressions in SK-N-SH cells were examined by FCM and real-time PCR. Results： FCM results showed that SH-N-SK cells expressed high level of Foxp3. The sensitive dosage of CTX on SK-N-SH cells was 6 mmol/L, and that of THP was 80 ng/ml. CTX （6 mmol/L） , THP （80 ng/ml） alone or in combination could not inhibit the expression of Foxp3 in SK-N-SH cells （P 〉 0.05）. Real-time PCR data also showed that CTX, THP alone or in combination could not down-regulate the expression of Foxp3 in SK-N-SH cells at mRNA level （P 〉 0. 05 ）. Conclusion ： Neuroblastoma SK-N-SH cells can express high level of Foxp3, but Foxp3 shows no chemosensitivity to CTX and THP.

关 键 词：FOXP3 成神经细胞瘤 SK—N—SH细胞 环磷酰胺 吡柔比星 

分 类 号：R971.1[医药卫生—药品]