La(C_7H_5O_3)_2·(C_9H_6NO)抑制Hela细胞生长作用机制研究  

Study on the inhibitory mechanism of La(C_7H_5O_3)_2·(C_9H_6NO) in the proliferation of Hela cells

作　　者：张辉[1] 喻莉萍[2] 李旭[1] 胡吉林[1] 何志雄[3] 林应标[3] 刘巧突[3] 李强国[1] 

机构地区：[1]湘南学院化学与生命科学系药学教研室,湖南郴州423000 [2]湘南学院图书馆,湖南郴州423000 [3]湖南省郴州市第一人民医院检验科,湖南郴州423000

出　　处：《中国医药导报》2010年第15期5-7,共3页China Medical Herald

基　　金：湖南省科技厅科技计划项目资助项目(NO.2008FJ4186);湖南省自然科学基金资助项目(NO.08JJ3014/B030805);国家自然科学基金资助项目(NO.20973145/B0309);湖南省教育厅科技处资助项目(NO.04C635)

摘　　要：目的:观察La(C7H5O3)2·(C9H6NO)对体外培养的Hela细胞的生长抑制作用,并进一步探讨其作用机制。方法:应用MTT法检测细胞增殖活性,流式细胞仪检测细胞周期分布,Annexin Ⅴ-FITC染色法检测细胞凋亡率。结果:不同浓度的La(C7H5O3)2.(C9H6NO)处理Hela细胞24～96h后,细胞增殖受到显著抑制,并呈浓度及时间依赖性;0.5、1.0、5.0、10.0μmol/L La(C7H5O3)2·(C9H6NO)处理72h后,Hela细胞G0/G1期细胞数量显著增加,S期细胞数量显著减少(P<0.01);各浓度组细胞凋亡率均显著增加(P<0.01)。结论:La(C7H5O3)2·(C9H6NO)对Hela细胞具有生长抑制作用,其机制与阻滞细胞周期及诱导凋亡有关。Objective： To investigate the inhibitory effect of La（C7H5O3）2·（C9H6NO） in the proliferation of Hela cells, and explore its mechanism. Methods： MTr assay, flow cytometry and Annexin V-FITC staining were respectively performed to determine the cell proliferation activity, cell cycle and apoptotie rate. Results： After treated with different concentrations of La（C7H5O3）2·（C9H6NO） for 24 to 96 hours, the proliferation activity of Hela cells was inhibited in a dose- and time-dependent manner. Hela cells were treated with 0.5, 1.0, 5.0, 10.0 μmol/L of La（C7H5O3）2·（C9H6NO）, the number of cells in G0/G1 phase significantly increased and the number of cells in S phase significantly increased in cells treated with different concentrations of La（C7H5O3）2·（C9H6NO） （P〈0.01）. Conclusion： La（C7H5O3）2·（C9H6NO） can inhibit the growth of Hela cells by blocking cell cycle and inducing apoptosis.

关 键 词：La(C7H5O3)2·(C9H6NO) HELA细胞 凋亡 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]