α-噻吩甲酰三氟丙酮-哌啶铈对DNA和磷酸二酯键模型物(BDNPP)的作用研究  

The studies about complex Ce(Ⅲ)with 2-thenoyltrifluoroacetone-piperidine ligands interacting on DNA,BDNPP

作　　者：张改清[1,2] 霍方俊[3] 阴彩霞[4] 杨频[4] 

机构地区：[1]吕梁高等专科学校化学化工系,山西吕梁033000 [2]山西大学分子科学研究所,山西大学化学生物学与分子工程教育部重点实验室,山西太原030006 [3]山西大学应用化学研究所,山西太原030006 [4]山西大学分子科学研究所山西大学化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《化学研究与应用》2010年第5期613-616,共4页Chemical Research and Application

基　　金：国家自然科学基金(20801032)资助项目;山西省自然科学基金(2009021006-2)资助项目;山西省归国基金2008资助;山西省优秀青年学术带头人专项基金资助项目

摘　　要：CeTTA4·HP(here,TTA=2-Thenoyltrifluoroacetone,HP=Piperidine)has been synthesized and characterized.we found that the CeTTA4·HP complex can cleave circular plasmid pBR322 DNA at pH7.2 and 37℃.In addition,we choose BDNPP(bis(2,4-dinitro phenyl)-phosphate)as a model compound to further study the cleavage mechanism for pBR322 DNA.From the second-order kinetics equation,we prove indirectly the mechanism may be hydrolytic cleavage.CeTTA4·HP（here,TTA=2-Thenoyltrifluoroacetone,HP=Piperidine）has been synthesized and characterized.we found that the CeTTA4·HP complex can cleave circular plasmid pBR322 DNA at pH7.2 and 37℃.In addition,we choose BDNPP（bis（2,4-dinitro phenyl）-phosphate）as a model compound to further study the cleavage mechanism for pBR322 DNA.From the second-order kinetics equation,we prove indirectly the mechanism may be hydrolytic cleavage.

关 键 词：CeTTA4.HP DNA切割 水解方式 

分 类 号：O641.4[理学—物理化学]