高效液相色谱法测定贯叶连翘中的4种成分  

HPLC simultaneous determination of four ingredients in Hypericum perforatum L.

作　　者：唐静[1] 薛洪宝[1] 刘海峰[1] 杨甲甲[1] 李晖[1] 

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《化学研究与应用》2010年第5期634-638,共5页Chemical Research and Application

摘　　要：本文利用高效液相色谱法同时测定中药贯叶连翘中绿原酸、芦丁、金丝桃苷和槲皮素四种成分的含量。采用Tiahhe Kromasil C18 100 A（5μm,250mm×4.6 mm）反相色谱柱;流动相为V（乙腈）∶V（水）=20∶80（含0.02%三氟乙酸）;紫外检测波长270nm;流速1.0 ml/min;柱温40℃。绿原酸、芦丁、金丝桃苷和槲皮素线性范围分别为3.4~34μg.mL-1（r=0.9993）,1.8~18μg.mL-1（r=0.9998）,2.3~23μg.mL-1（r=0.9999）,3.5~35μg.mL-1（r=0.9991）,平均回收率（n=5）分别为98.4%（RSD=1.48%）,101.8%（RSD=0.74%）,103.7%（RSD=0.77%）,103.5%（RSD=1.28%）。方法线性范围宽、相对标准偏差低、精密度高、重现性好,应用于贯叶连翘及制剂样品的测定,结果令人满意。A simple method to separate and quantify simultaneously four ingredients（chlorogenic acid,rutin,hyperin and quercetin） in traditional Chinese medicine Hypericum perforatum L.was established by high performance liquid chromatography（HPLC）.An Tiahhe Kromasil C18 100A（5μm,250mm×4.6mm） reversed-phase column was applied at 40℃.The wavelength of ultraviolet detector was set at 270nm,and mobile phase was double-distilled water contained 20% acetonitrile（V/V） and 0.02% trifluoroacetic acid（TFA） with a flow rate of 1.0 ml/min in the performance.The linear ranges of chlorogenic acid,rutin,hyperin and quercetin were 3.4～34μg·mL-1（r=0.9993）,1.8～18μg·mL-1（r=0.9998）,2.3～23μg·mL-1（r=0.9999）,3.5～35μg·mL-1（r=0.9991）,respectively;the average recoveries were 98.4%（RSD=1.48%）,101.8%（RSD=0.74%）,103.7%（RSD=0.77%）,103.5%（RSD=1.28%）,respectively.This method had a wide linear range,low relative standard deviation,high precision and perfect reproducibility,and obtained satisfactory results in the determination of Hypericum perforatum L.and its preparation.

关 键 词：贯叶连翘 同时定量 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]