应用玻璃化冷冻技术保存人未成熟卵母细胞的研究  

Study on vitrification of human immature oocytes

作　　者：蒋晓明[1] 沙爱国[1] 周友泉[1] 李萍[1] 

机构地区：[1]中国人民解放军第174医院生殖医学中心,厦门361003

出　　处：《中国优生与遗传杂志》2010年第5期115-116,58,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的利用玻璃化冷冻保存技术合理利用常规促排卵周期获得的未成熟卵母细胞。方法 1.对注射hCG后38h、43h、60h获得GV期卵母细胞进行玻璃化冷冻保存,冻融后的未成熟卵母细胞行体外成熟培养(IVM),用ICSI技术对获得的成熟卵母细胞完成授精,并体外胚胎培养至囊胚期。比较各组间的冻融存活率、体外成熟率、受精率、卵裂率及囊胚形成率。2.对GV卵分别行先玻璃化冻融后IVM和先IVM后玻璃化冻融,比较它们的冻融存活率、体外成熟率、受精率、卵裂率及囊胚形成率。结果 1.38h与43h卵龄GV卵在各项指标比较上没有差异,60h卵与前2组有相似的冻融存活率,但在体外成熟率、受精率和卵裂率上较前2组出现明显下降趋势。3组均无囊胚形成。2.先玻璃化冻融后IVM和先IVM后玻璃化冻融在未成熟卵母细胞的利用率上没有差异。结论 1.冷冻卵龄较短的GV期卵母细胞能获得更好的冷冻保存效果。2.玻璃化冻融和IVM先后不影响未成熟卵母细胞的利用。Objective： To set up an efficient method for utilization of immature oocytes retrieved from routine COH with vitrification. Methods： 1. The oocytes of GV stage retrieved in 38, 43, and 60 hours after hCG injection were vitrificated. After thawing, the immature oocytes were subjected to IVM. ICSI was applied for insemination of the obtained mature oocytes. The rates of survival, maturation, fertilization, cleavage and blastocyst were compared. 2. The rates of survival, maturation, fertilization, cleavage and blastocyst obtained both from the procedure of vitrification before IVM or IVM before vitirficaion were compared. Results： 1. There were no differences between group 38 and 43 hours in each compared rate. The rates of maturation, fertilization and cleavage obviously lowered in group 60 hours. No blastocyst appeared in each group. No differences were found in both procedure of cryopreservation before IVM or IVM before cryopreservaton. Conclusion： It was better to cryopreserve the GV oocyte retrieved in 38 and 43 h after hCG injection. The availability was not different both in the procedure of dryopreservation before IVM or IVM before cryopreservaton.

关 键 词：人未成熟卵母细胞 玻璃化冷冻 

分 类 号：R714.8[医药卫生—妇产科学]