甘薯DNA提取及ISSR反应体系的建立  

DNA Extraction and Establishment of Sweetpotato ISSR Reaction System

作　　者：宋吉轩[1,2,3] 王季春[1] 雷尊国 黄团 

机构地区：[1]西南大学农学与生物科技学院,重庆400715 [2]贵州省生物技术研究所,贵阳550006 [3]贵州省生物技术重点实验室,贵阳550006

出　　处：《种子》2010年第4期40-43,共4页Seed

基　　金：贵州省科学技术基金[黔科合J字(2009)2094]

摘　　要：以甘薯幼叶为试验材料,对甘薯基因组DNA的提取,并对其纯度、浓度进行检测,结果表明,DNA扩增效果良好,完全能满足进一步试验的需要。同时对甘薯ISSR-PCR反应体系中各个影响因素进行了优化,建立了适合甘薯ISSR分子标记的最佳反应体系,即在20μl的反应体系中,含稀释浓度为10倍的DNA模板,0.30 mmol/L dNTP,1.00μmol/L引物,1.00 UTaq酶,0.5%去离子甲酰胺比较适合甘薯DNA的特异扩增。Extracting DNA from young leaves of sweet potato,and the purity,concentration of the DNA were tested.The results showed that the DNA could be well amplified,which was fully able to meet the demand of further experiments.At the same time,impacting factors of sweet potato ISSR-PCR reaction system were optimized,and established the best suitable reaction system for sweet potato.That was in 20 μl reaction system,DNA template with 10 times of dilution concentration,0.30 mmol/L dNTP,1.00 μmol/L primerand 1.00 U Taq enzyme and 0.5% isopropanol.

关 键 词：甘薯 DNA提取 ISSR反应体系 建立 

分 类 号：S531[农业科学—作物学]