转hpa1_(Xoo)基因棉花悬浮细胞培养及其对黄、枯萎病菌的抗性  

The Culture of the Suspension Cell of Transgenic hpa1_(Xoo) Cotton and Its Resistance to Verticllium dahliae and Fusarium oxysporum f. sp. vasinfectum

作　　者：缪卫国[1] 倪萌[2] 宋从凤[1] 王金生[1] 

机构地区：[1]南京农业大学植物保护学院农业部作物病虫害监测与防控重点开放实验室,南京210095 [2]新疆农业大学,乌鲁木齐830052

出　　处：《西北农业学报》2010年第1期44-48,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金项目(30230240);国家重点基础研究发展规划资助项目(G2000016201);国家科技部项目(2004BA901A36)

摘　　要：为验证对黄萎病菌(Verticllium daliae)、枯萎病菌(Fusarium oxysporum f.sp.vasinfectum)的早期抗性反应,确定了转hpa1Xoo基因棉花株系T-33悬浮细胞适宜的培养条件,并成功获得了T-33和其出发品种Z35的悬浮细胞。以1:20的比例,将T-33悬浮细胞与黄、枯萎菌分生孢子液分别共培养0～24h,发现T-33悬浮细胞在0～6h对两种病原菌的抗性能力较强,悬浮细胞死亡率显著低于Z35(P<0.05〉。与Z35相比,在3h和6h时,针对棉花黄萎病菌T-33悬浮细胞死亡率分别低52%和35%,针对枯萎病菌分别低42%和25%。说明hpa1Xoo编码的harpinXoo表达赋予转基因棉花T-33悬浮细胞系对两种病原菌的抗性高于其出发品种Z35。For confirming the earlier resistance reaction,the suspension cells of transgenic hpa1Xoo cotton plant T-33 and untransformed cotton plant Z35 were cultured successfully,and the suspension cell of T-33 plant were mixed and co-cultured with Verticllium daliae and Fusarium oxysporum f.sp.vasinfectum conidia at 1：20 ratio for 0～24 h respectively.Z35 was a control.The death rate of the suspension cell of T-33 was significantly lower than that of Z35 during 3～12 h culture time,in particular reduced 52% at 3 h and 35% at 6 h to V.dahliae,and reduced 42% at 3 h and 25% at 6 h to F.oxysporum f.sp.vasinfectum.The evidence indicated the expression of harpinXoo encoded by hpa1Xoo enhanced the resistance of T-33 suspension cells to two pathogens compared with Z35 suspension cells.

关 键 词：harpinXoo 转基因棉花 悬浮细胞 黄萎病菌 枯萎病菌 

分 类 号：S562[农业科学—作物学]