抗坏血酸过氧化物酶基因转化苹果的研究  

Agrobacterium-mediated Transformation of Gala Apple with APX Gene

作　　者：李颖[1,2] 马锋旺[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]西藏自治区农科院中心实验室,拉萨850002

出　　处：《西北农业学报》2010年第1期140-143,163,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西北农林科技大学"拔尖人才支持计划"项目

摘　　要：利用根癌农杆菌介导法对"嘎啦"苹果叶片进行遗传转化,获得"嘎啦"苹果的转基因植株。2d的预培养,OD600值约为0.6的工程菌液浓度,侵染时间10min,3d的共培养,延迟筛选10d获得较高的转化效率。PCR检测和Gus染色表明,APX基因已整合到苹果基因组中。The transgenic Gala apple plants with APX gene by Agrobacterium tumefacien were obtained.The results showed that highest transformation frequency was obtained when the leaves was precultivated 2 days,the OD600 of Agrobacterium was about 0.6,the Agrobacterium infected 10 min,3 days coculture,and 10 days of detention screening.Results of PCR and Gus proved that the APX gene had integrated into apple genome.

关 键 词：苹果 根癌农杆菌 遗传转化 抗坏血酸过氧化物酶基因 

分 类 号：S661.1[农业科学—果树学]