HPLC法同时测定大鼠血浆中厄贝沙坦和吡格列酮的浓度  

Simultaneous Determination of Irbesartan and Pioglitazone in Rats Plasma by HPLC

作　　者：杜贯涛[1] 翁春梅[1] 刘广军[1] 苏丹[1] 陈桂林[1] 谢小菊[1] 

机构地区：[1]江苏常州市第二人民医院药剂科,常州市213003

出　　处：《中国药房》2010年第17期1557-1559,共3页China Pharmacy

基　　金：常州市科技局常州四药临床药学科研基金资助项目(CS2008903)

摘　　要：目的:建立同时测定大鼠血浆中厄贝沙坦和吡格列酮浓度的高效液相色谱法。方法:采用MERCKPurospherC18色谱柱,0.02mo·lL-1醋酸盐缓冲液(pH=3.6)-乙腈(60∶40)为流动相,检测波长为245nm,流速为1.0mL·min-1,柱温为35℃,进样量为40μL。结果:厄贝沙坦和吡格列酮检测浓度的线性范围分别为0.1～12μg·mL-1(r=0.9997)、0.1～12μg·mL-1(r=0.9998),萃取回收率大于75%,方法回收率大于90%,日内RSD小于4.4%,日间RSD小于8.1%。结论:该方法准确、灵敏、快速,可用于同时测定大鼠血浆中厄贝沙坦和吡格列酮的浓度。OBJECTIVE： To establish an HPLC method for simultaneous determination of irbesartan and pioglitazone in rat plasma. METHODS： The separation was performed on MERCK Purospher C18 column with mobile phase consisted of 0.02 mol·L-1 acetate buffer （pH=3.6）-acetonitrile （60 ∶ 40）. The detection wavelength was set at 245 nm and the flow rate was 1.0 mL·min-1. The column temperature was kept at 35 ℃ and injection volume was 40 μL. RESULTS： The linear ranges of irbesartan and pioglitazone were 0.1～12 μg·mL-1 （r=0.999 7） and 0.1～12 μg·mL-1 （r=0.999 8） respectively. The extraction recovery and method recovery of the two drugs were more than 75% and 90% respectively. Intra-day RSD was lower than 4.4% and inter-day RSD was lower than 8.1%. CONCLUSION： The method is accurate, sensitive and rapid for the simultaneous determination of irbesartan and pioglitazone in rat plasma.

关 键 词：高效液相色谱法 厄贝沙坦 吡格列酮 血药浓度 大鼠 

分 类 号：R969.1[医药卫生—药理学] R972.4[医药卫生—药学]