藏猪生长激素基因(pGH)多态性研究  

Polymorphisms of the Porcine Growth Hormone Gene (pGH) in Tibet Pig

作　　者：张浩[1] 强巴央宗[2] 张明明[3] 彭俊飞[1] 杨涛[2] 达娃卓玛[2] 吴常信[1] 

机构地区：[1]中国农业大学动物科技学院,北京100094 [2]西藏农牧学院动物科学系,西藏林芝860000 [3]安徽省农业科学院情报研究所,合肥230031

出　　处：《西北农业学报》2010年第2期15-19,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家科技支撑项目(2008BADB2B01);西藏自治区科技计划项目

摘　　要：猪生长激素基因(pGH)是猪生长发育性状重要的候选基因之一。采用PCR-RFLP技术检测藏猪pGH基因+159^+734 bp片段的DraI、ApaI和MspI酶切多态性。结果显示,藏猪群体中DraI酶切全部切开,无多态;ApaI酶切具有4个等位基因,出现5种基因型,其中CC基因型(299A/432C)频率最高,为0.7857;藏猪群体MspI酶切出现3种基因型,杂合性CT基因型频率最高,为0.5089,等位基因C和T频率分别为0.5580和0.4420。经比较,藏猪pGH基因DraI酶切位点与其他猪种一样无多态性,而ApaI酶切出现了特异的432突变位点,MspI酶切等位基因分布相对均衡,表现出与其他猪种的差异。The porcine growth hormone（pGH） gene is one of important candidate for development and growth trait.In the present study,the polymorphism of fragment ＋159～＋734 of pGH gene was measured with the methods of PCR-RFLP by restriction endonuclease Dra I,Apa I and Msp I.The results showed that the Tibet pig has no diversity for PCR-Dra I-RFLP of pGH gene.There are four alleles and five genotypes for PCR-Apa I-RFLP of pGH gene,and the genotype CC（299A/432C） is predominant（0.7857）.The Tibet pig population has three genotypes for PCR-Msp I-RFLP of pGH gene,and the predominant genotype CT is（0.5089）.The frequencies of allele C and allele T are 0.5580 and 0.4420 respectively.By comparing with other breeds,the Tibet pig has differential genotypes for Apa I,and the distribution of alleles for Msp I is uniform relatively,which may be the difference of Tibet pig with other pig breeds.

关 键 词：藏猪 pGH基因 PCR-RFLP 多态性 

分 类 号：S828.2[农业科学—畜牧学]