奶牛乳房炎病原菌的分离鉴定及耐药性分析  

Isolation,Identification and Drug Sensitive Test of Pathogenic Bactcria Causing Dairy Cattle Mastitis

作　　者：倪春霞[1,2] 蒲万霞[3] 胡永浩[1] 邓海平[3] 王玲[3] 孟晓琴[3] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,新兽药工程重点开放实验室,兰州730050 [3]中国农业科学院兰州畜牧与兽药研究所新兽药工程重点开放实验室,兰州730050

出　　处：《西北农业学报》2010年第2期20-24,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家支撑计划项目“奶牛主要疾病综合防控技术研究(2006BAD04A05-04)”

摘　　要：为弄清中国不同地区奶牛乳房炎主要病原菌种类、分布情况及其药物敏感特性,并为奶牛乳房炎的综合防治提供依据,对在内蒙古、甘肃、四川和上海4地部分奶牛场采集的472份临床型或亚临床型乳房炎乳样进行细菌分离鉴定,并对主要病原菌进行药敏试验。结果表明,检测奶样中检出细菌482株,其中金黄色葡萄球菌149株,占30.91%;表皮葡萄球菌91株,占18.88%;无乳链球菌60株,占12.45%;停乳链球菌60株,占12.45%;乳房链球菌35株,占7.26%;大肠杆菌33株,占6.85%。各个地区优势菌群存在差异,药敏试验结果也不相同,分离出的细菌绝大部分对环丙沙星、左氟沙星、氧氟沙星和先锋霉素V高度敏感,但对青霉素、红霉素和万古霉素有较强耐药性。To study the the species and distributing of the main pathogenic bacteria causing dairy cow mastitis in different regions of China,identify the susceptivity to antibiotic of mail pathogens,and to provide a theorical foundation for comprehensive prevention and control of dairy cow mastitis.472 milk samples from cows suffered from clinical or subclinical mastitis were collected from cattle farm in Inner Mongolia,Gansu,Sichuan and Shanghai,and the main pathogens were identified and then used for drug sensitive test.The results showed that 482 strains of bacteria were isolated from the milk samples,149 strains among them are Staphlococcus aureus（30.91 %）,91 are Staphylococcus epidermidis（18.88%）,60 are Streptococcus agalactiae（12.45%）,60 are Streptococcus dysgalactiae（12.45%）,35 are Streptococcus uberis（7.26%）,33 are E.coli（6.85%）.The main pathogenic bacteria of each area is dissimilar,most of which were hyper sensitive to Ciprofloxacin,Levofloxacin,Ofloxacin,Cefazolin,but were completely not sensitive to Benzylpenicillin,Erythromycin and Vancomycin.

关 键 词：奶牛乳房炎 病原菌 分离鉴定 药敏试验 

分 类 号：S858.23[农业科学—临床兽医学]