大白菜杂交种“冠春”特异性SCAR标记的获得及其验证  

Two Special SCAR Markers of Guanchun,a Heading Spring Chinese Cabbage (Brassica campestris syn. rapa L. ssp. pekinensis (Lour) Olsson) Hybrid,and Its Verification

作　　者：张鲁刚[1] 王绮[1] 张战凤[1] 惠麦侠[1] 张明科[1] 

机构地区：[1]西北农林科技大学园艺学院农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌712100

出　　处：《西北农业学报》2010年第2期177-182,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：公益性行业科研专项资助(nyhyzx07-007);国家科技支撑计划(2006BAD01A7-1-03)资助

摘　　要：以获得的春大白菜品种"冠春"的特异性RAPD标记为依据,通过对8个特异片段的回收,克隆测序,采用引物延长法设计了SCAR引物,经过双亲及其杂交一代的鉴定,6个SCAR引物多态性消失,2个SCAR引物扩增出了单一的特异条带,而且SCAR标记的差异与原始的RAPD特异性标记的差异完全相同,为大白菜杂交种"冠春"的品种鉴定和品种保护提供了技术依据。In order to get a stable DNA marker for hybrid F1 identification,the DNA segments of the special RAPDs obtained before were recovered,cloned and sequenced,then eight pairs of SCAR primers were designed and verified in F1 and two parents,its results showed that six SCAR primers lost the RAPD polymorphic and two SCAR primers amplified a single special SCAR marker,which difference is in accord with the RAPD.Therefore,a stable and simple method for the Guanchun F1 identification was established.

关 键 词：大白菜 冠春 杂交种 种子纯度 SCAR 

分 类 号：S634.1[农业科学—蔬菜学]