血管活性肠肽在动情周期大鼠子宫中的分布  

Distribution of Vasoactive Intestinal Peptide in Uterus of Rats during Estrus Cycle

作　　者：郝建军[1] 徐永平[1] 逄国梁 李君锦[1] 郭翠香[1] 王震[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]陕西省畜牧技术推广总站,西安710016

出　　处：《西北农业学报》2010年第3期40-44,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省奶牛乳房炎技术支撑与示范项目(14220201);西北农林科技大学科研专项项目(06ZR003)

摘　　要：采用免疫组化SP法,观察血管活性肠肽(VIP)免疫反应阳性物质在SD大鼠动情周期子宫中的分布情况和变化规律。结果表明,在内膜层中,VIP免疫阳性产物定位于子宫内膜上皮细胞、腺上皮细胞、基质细胞和血管内皮细胞中。在肌层中,VIP免疫阳性产物主要位于VIP样阳性神经纤维中。VIP免疫阳性产物相对表达量的t检验表明:子宫内膜VIP在动情后期表达量较发情前期极显著减少(P<0.01);与动情前期相比,子宫肌层VIP在动情期的表达量显著减少(P<0.05),在动情后期的表达量极显著减少(P<0.01)。但是在动情间期VIP的表达量较动情后期又极显著回升(P<0.01)。VIP在动情周期大鼠子宫中表达分布存在一定规律性,可能与其免疫调节和腺体分泌等功能有关。Using Streptavidin-peroxidase（SP）to observe the distribution and expression variation of vasoactive intestinal peptide（VIP）in uterus of the sprague dawley during estrus cycle.The results showed that,in endometrium layer,the VIP immunoreactive products mainly located in endometrial epithelial cells,the glandular epithelium,stromal cells and vascular endothelial cells.In myometrium layer,the VIP immunoreactive products mainly located in VIP immunoreactive nerve fibers.T-test result of the relative expression of immunoreactive product VIP showed that,in contrast with the Proestrus,expression of VIP in metestrus of endometrium was extremely significantly decreased（P0.01）,expression of VIP in Estrus of myometrium was significantly decreased（P0.05）,expression of VIP in Metestrus was extremely significantly decreased（P0.01）.But in Diestrus,contrasted with the metestrus,expression of VIP was extremely significantly increased（P0.01）.The results indicated that the expression of VIP in the uterus in Estrus cycle was found to adapt to some certain rules,and might relate to its immunoregulation and gland secretion functions.

关 键 词：血管活性肠肽 免疫组织化学SP法 动情周期 子宫 SD大鼠 

分 类 号：R339.2[医药卫生—人体生理学]