1,25(OH)_2D_3对巨噬细胞吞噬金黄色葡萄球菌过程的影响  

EFFECT OF 1,25-DIHYDROXYVITAMIN D_3 ON THE PROCESS OF PHAGOCYTIZING STAPHYLOCOCCUS AUREUS IN MACROPHAGES

作　　者：丁晓飞[1] 张青丽[2] 郝莉[1] 李冰燕[1] 张增利[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,苏州215123 [2]新疆医科大学第五附属医院,乌鲁木齐830011

出　　处：《营养学报》2010年第2期130-132,137,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30570883);中国博士后科学基金(No.20070410767)

摘　　要：目的研究维生素D(VD)对巨噬细胞吞噬金黄色葡萄球菌的吞噬活性及吞菌后巨噬细胞自身凋亡过程的影响。方法巨噬细胞株RAW264.7分为对照组(C),细菌组(B),活性VD+细菌组(VD+B),VD+B组加入终浓度为10-8mol/L活性VD培养24h后,与细菌组一起感染金黄色葡萄球菌1h,用流式细胞仪测定各组巨噬细胞吞噬率、线粒体膜电位、细胞内游离钙离子浓度、活性氧的差异。结果VD+B组巨噬细胞吞噬金黄色葡萄球菌的能力显著高于B组;VD+B组巨噬细胞吞菌后线粒体膜电位显著低于B组;同时,VD+B组的细胞内游离钙离子浓度显著低于B组的;两组间活性氧的生成没有显著性差异。结论维生素D能促进巨噬细胞对金黄色葡萄球菌的吞噬能力,对于吞噬细菌后巨噬细胞自身的凋亡有一定抑制作用。Objective To investigate the effect of vitamin D （VD） on macrophage to phagocytize Staphylococcus aureus （SA）. Method Macrophoge cell lines RAW264.7 were allocated into 3 groups：control group（C）,bacterium group（B）,active vitamin D＋ bacterium group （VD＋B）. Cells in the VD＋B group were incubated with 10^-8mol/L active vitamin D for 24h,then cells in the VD＋B group and the B group were cultured with SA for 1h,and phagocytosis rate,mitochondrial membrane potential,[Ca～^（2＋）]i,reactive oxygen species were determined by flow cytometry （FCM）. Results The phagocytizing activity of macrophage in VD＋B group was significantly higher than that in B group 1h after infection,At the same time,the mitochondrial member potential and [Ca～（^2＋）]i of macrophage in VD＋B group were distinctly lower than that in B group; but reactive oxygen species of macrophage in the VD＋B group was insignificantly different from B group. Conclusion Vitamin D can reinforce the phagocytizing activity of macrophage and inhibit the apoptosis of macrophage after phagocytize Staphylococrcus aureus.

关 键 词：维生素D 巨噬细胞 金黄色葡萄球菌 吞噬作用 细胞凋亡 

分 类 号：R378[医药卫生—病原生物学]