应用生物反应器建立人用冻干狂犬病疫苗(Vero细胞)生产工艺的研究  

Bioreactor Application to Establish Production Technology of Freeze-Dried Rabies Vaccine (Vero Cells ) for Human Use

作　　者：李志强[1] 袁延宝[1] 郑淑媛[1] 代长海[1] 张夫坤[1] 顾丹阳[1] 戚凤春[1] 

机构地区：[1]长春生物制品研究所,吉林长春130062

出　　处：《微生物学杂志》2010年第1期96-99,共4页Journal of Microbiology

摘　　要：应用15L生物反应器,采用片状载体对Vero细胞进行高密度培养、制备高毒力滴度的狂犬病毒收获液,经纯化后生产人用冻干狂犬病疫苗。采用15L生物反应器对培养方法(批次培养和连续灌流培养)进行试验,收获高毒力滴度的狂犬病毒收获液并制备人用冻干狂犬病疫苗。结果表明:Vero细胞在接种狂犬病毒后可以连续收获病毒液达到25d以上,冻干狂犬病疫苗的效价可以达到5.54IU/剂。本工艺可以用于进行大规模的人用冻干狂犬疫苗的生产。15 L bioreactor was applied adopting chip carrier to carry out high density cultivation of Vero cells and prepare high titer rabies virus harvested in liquid and produce freeze-dried rabies vaccine for human use after purification.Rabies virus was first cultured adopting 15 L bioreactor by batch culture and continuous flow culture as a trial of cultivation method,and the culture procedure was optimized,based on which high titer rabies virus liquid was harvested to prepare freeze-dried rabies vaccine for human use.After inoculated with rabies virus Vero cells could harvest virus liquid continuously for more than 25 d,and the valence of prepared freeze-dried rabies vaccine reached as high as 5.54 IU/dose.The developed technology can be used for large-scale production of freeze-dried rabies vaccine for human use.

关 键 词：生物反应器 片状载体 冻干狂犬病疫苗 

分 类 号：R392.7[医药卫生—免疫学]