黄柏饮片NACE-DAD指纹图谱的研究  

Studies on NACE-DAD Fingerprint of Cortex Phellodendri

作　　者：李俊松[1] 刘训红[1] 蔡宝昌[1] 傅兴圣[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,江苏南京210029

出　　处：《中药材》2010年第3期349-352,共4页Journal of Chinese Medicinal Materials

基　　金：江苏省中药炮制重点实验室开放式课题(ZYPZ007)

摘　　要：目的:建立黄柏饮片NACE-DAD指纹图谱分析方法,评价不同产地、种属黄柏饮片的质量。方法:选择非水毛细管电泳分离模式,以40 mmol/L乙酸钠-40 mmol/L乙酸铵-无水甲醇缓冲液(pH5.8)为运行缓冲液,分离电压为25 kV,以小檗碱为参照物(IS),测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以9个共有峰为特征指纹信息的NACE-DAD指纹图谱;发现少数产地黄柏饮片的指纹图谱有一定差异,生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论:方法准确可靠,重现性好,可作为黄柏饮片内在质量评价的依据。Objective：To establish the analytical method for the fingerprint of Cortex Phellodendri by NACE-DAD and estimate the quality of Cortex Phellodendri from different habitats and species.Methods：Based on the mode of nonaqueous capillary electrophoresis,40 mmol/L sodium acetate and 40 mmol/L ammonium acetate methanol solution was selected for the buffer（pH 5.8）.The separation voltage was 25 kV and detection wavelength was set at 210 nm.Berberine was used as a reference standard,the chromatographic fingerprint was determined.The data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples.Results：NACE-DAD fingerprints with 9 commonpeaks were established preliminarily.It was discoveried that a small number of samples differed from others.Regarding to the fingerprints of Cortex Phellodendri and its processed products,there were obvious differences in the relative areas of commonpeaks.Conclusion：The method is reliable,accurate and can be used for quality control of Cortex Phellodendri.

关 键 词：黄柏 非水毛细管色谱二极管阵列检测法 指纹图谱 

分 类 号：R283[医药卫生—中药学] R284.1[医药卫生—中医学]