加味三棱丸抗子宫内膜异位症雌激素生成作用及对内膜细胞凋亡的影响  

Inhibitory Effect of Jiawei Sanleng Pill Medicated Serum on Estrogen Production and Its Influence on Apoptosis of Human Endometrial Cells of Endometriosis

作　　者：李傲[1] 汪莹[2] 徐晓玉[3] 

机构地区：[1]重庆理工大学化学与生物工程学院,重庆400050 [2]重庆医科大学中医药学院,重庆400016 [3]西南大学药学院与中医药学院,重庆400716

出　　处：《中药材》2010年第3期401-406,共6页Journal of Chinese Medicinal Materials

基　　金：重庆市科委应用基础研究项目[(2002)18号-99]

摘　　要：目的:研究加味三棱丸(SLW)对子宫内膜异位症(内异症)雌二醇(E2)生成的抑制作用,并探讨其诱导内异症内膜细胞凋亡的机制。方法:培养子宫肌瘤和内异症患者在位子宫内膜细胞,以子宫肌瘤内膜细胞为对照,内异症在位内膜细胞经含SLW大鼠血清高、中、低剂量处理后,分别在不同的时间点采用电化学发光免疫法检测E2的分泌水平;流式细胞术和TUNEL法检测细胞的早期和晚期凋亡率;通过时效关系考察,明确SLW诱导内异症在位内膜细胞早、晚期凋亡的最佳时间后,重新将培养的内膜细胞分为6组:子宫肌瘤组、内异症组、内异症+SLW血清中剂量组、内异症+阿那曲唑血清组、内异症+SLW血清中剂量+雌二醇组和内异症+雌二醇组,在最佳时间点检测各组细胞的早期和晚期凋亡率。结果:SLW可显著降低内异症在位内膜细胞分泌E2的水平,并呈浓度和时间依赖性。子宫肌瘤组内膜细胞的早、晚期凋亡百分率随时间延长而增加,并显著高于同时间点的内异症组(P<0.05或P<0.01);经SLW血清中剂量处理72、96 h后,内异症在位内膜细胞的早、晚期凋亡百分率分别为(35.67±1.73)%和(57.56±1.09)%,较其他处理时间的诱导作用显著;当SLW血清中剂量与雌二醇5.30 ng/L联合处理72 h或与雌二醇6.36 ng/L联合处理96 h后,细胞的早、晚期凋亡百分率分别降低至(11.25±2.00)%和(7.72±0.71)%,与SLW血清中剂量比较,差异显著(P<0.01),添加的雌二醇浓度未能完全抑制SLW诱导内异症内膜细胞的凋亡;单独给予雌二醇5.30、6.36 ng/L进行处理,内异症内膜细胞的早、晚期凋亡百分率有进一步降低的趋势,但无统计学意义(P>0.05),芳香化酶抑制剂阿那曲唑与雌二醇的作用恰好相反。结论:SLW可能通过降低内异症在位内膜细胞分泌雌二醇的水平,发挥其诱导内膜细胞凋亡的作用。Objective：To study the inhibitory effect on estradiol（E2） production of endometriosis by medicated serum of Jiawei Sanleng pill（SLW） and approach the mechanism of inducing apoptosis of human endometrial cells of endometriosis by medicated serum of SLW.Methods：The eutopic endometrial cells of hysteromyoma and endometriosis patients were cultured.Taking that of the endometrial cells of hysteromyoma as control,the secretion level of E2 of endometrial cells in the culture media supernatant at different time point with the treatment of high,middle and low dose of SLW serum was detected by electrochemiluminescence immunoassay and the apoptosis percentage of endometrium cells in early stage and late stage was detected by flow cytometry and TUNEL method.After the optimal time for SLW to induce apoptosis of eutopic endometrial cells of endometriosis in early stage and late stage was identified based on time-effect relationship,another endometrium cells were divided into six groups：hysteromyoma endometrium group,eutopic endometrium of endometriosis group,eutopic endometrium of endometriosis＋middle dose of SLW serum group,eutopic endometrium of endometriosis＋anastrozole serum group,eutopic endometria of endometriosis＋middle dose of SLW serum＋E2 group and eutopic endometrium of endometriosis＋E2 group.The apoptosis percentage of endometrial cells in early stage and late stage of each group was detected according to the optimal time point respectively.Results：The secretion level of E2 of eutopic endometrium of endometriosis was decreased by SLW,which showed the dependence of concentration and time.The apoptosis percentage of endometrial cells in hysteromyoma endometrium group in early stage and late stage was increased along with the time and they was significantly higher than that of eutopic endometrium of endometriosis group at the same time point（P〈0.05 or P〈0.01）.After 72 hours and 96 hours,with the treatment of middle dose of SLW serum,the apoptosis percentage of eutopic endometrial cells

关 键 词：子宫内膜异位症 加味三棱丸 雌二醇 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]