抗原肽浓度对初始T细胞向Th1／Tc1、Th2／Tc2分化影响的研究  

Peptide concentration regulates priming of naive T cells to develop into Th1/Tc1 or Th2/Tc2 cells

作　　者：梁华[1] 刘霜[2] 沈弢[3] 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫室,北京100050 [2]首都医科大学附属北京佑安医院 [3]北京大学医学部基础医学院微生物系,100191

出　　处：《中华微生物学和免疫学杂志》2010年第4期297-302,共6页Chinese Journal of Microbiology and Immunology

基　　金：科技部国际科技合作计划（2007DFC30230）;传染病预防控制国家重点实验室项目（2008SKLID101）;973计划（2005CB523103）

摘　　要：目的研究不同浓度抗原肽（OVA）对初始CD4^＋T细胞向Th1／Th2、初始CD8^＋T细胞向Tc1／Tc2分化偏向性的影响。方法采用系列浓度TCR特异性抗原肽与小鼠脾脏树突状细胞联合培养，然后刺激小鼠初始CD4^＋或CD8^＋T细胞。流式细胞术检测细胞内因子IFN-γ和IL4的表达水平，同时用CFSE检测活化T细胞的增殖水平。结果低浓度抗原肽刺激初始CD4^＋T向Th2、初始CD8^＋T向Tc2分化；而高浓度抗原肽刺激初始CD4^＋T向Th1、初始CD8^＋T向Tc1分化。Th细胞比Tc细胞受影响程度要明显。结论抗原肽在很大浓度范围内均可以刺激初始T细胞的活化，但随着浓度的升高，分化方向从Th2、Tc2逐渐向Th1、Tc1倾斜。这一结论为动物实验中疫苗免疫剂量的控制提供了重要的参考价值。Objective To study the effect of concentration of TCR-specific antigen peptides on priming naive CD4^＋ T cells to develop into Th1/Th2 cells or naive CD8^＋ T cells to develop into Tc1/Tc2 cells. Methods TCR-specific peptides with series of concentration were co-cultured with murine spleen DCs to activate murine naive CD4^＋/CD8^＋ T cells. The production of intracellular eytokines IFN-γ and IL-4 were measured by flow cytometry. The dividing profile of activated T cells was analyzed by CFSE staining. Results Lower concentration of specific peptides favored Th2/Tc2 polarization while higher concentration benefited Th1/Tc1 polarization. The influence of peptides concentration on Th cells differentiation is higher than that on Tc cells. Conclusion Antigen peptides can stimulate activation of naive T cells in a wide range of concentration. However, with the increase of peptides concentration, activated T cells differentiated grad- ually from Th2/Tc2 to Th1/Tc1, which will provide significant value to control immunized dose of vaccine candidates in animal experiments.

关 键 词：抗原肽 初始CD4^+/CD8^+细胞 树突状细胞 分化 

分 类 号：R392[医药卫生—免疫学]