慢性乙型肝炎患者细胞毒T细胞内穿孔素的表达与病毒载量的关系  

The relationship between cytotoxic T cells Perforin level and HBV DNA copies in patients with chronic hepatitis B

作　　者：刘波[1] 徐岳军[1] 庄晓玲[1] 鲍舟君[1] 方国安[1] 

机构地区：[1]浙江省舟山医院检验中心,316004

出　　处：《中华微生物学和免疫学杂志》2010年第4期315-317,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究慢性乙型肝炎患者细胞毒T细胞内穿孔素（Perforin）、干扰素（IFN-γ）和白细胞介素10（IL-10）表达与病毒消除的关系。方法对50例慢性乙型肝炎患者外周血进行短期培养后，利用流式细胞术测定CD8^＋细胞内Perforin、IFN-1和IL-10表达，并与30例正常对照进行比较；利用荧光定量PCR检测慢性乙型肝炎患者HBVDNA的载量，分析其与CD8^＋细胞内Perforin、IFN-1和IL-10表达的关系。结果慢性乙型肝炎组CD8^＋T细胞Perforin、IFN-1和IL—10的表达分别为（5．30±2．62）％、（4．05±2．25）％和（0．77±0．50）％，明显低于正常对照组（t分别为4．50、4．56、4．20，P均〈0．01）；26例HBeAg阳性的慢性乙型肝炎患者CD8^＋T细胞Perforin和IFN-1的表达分别为（4．54±1．93）％和（3．32±1．59）％，明显低于24例HBeAg阴性的慢性乙型肝炎患者（t分别为2．22、2．54，P均〈0．05）；慢性乙型肝炎患者CD8^＋T细胞内Perforin和IFN-γ表达率与乙肝病毒DNA载量成负相关（r分别为-0．539、-0．340，P〈0．01、P〈0．05）。结论慢性乙型肝炎患者细胞毒T细胞内Perforin、IFN-1和IL-10表达减少，可能与慢性乙型肝炎患者病毒长期存在，病程迁延不愈有关。Objective To analyze the relationship between cytotoxic T cells Pefforin level, IFN-γ and IL-10 in patients with chronic hepatitis B. Methods After a short-term cultivation of peripheral blood collected from 50 patients with chronic hepatitis B, a flow cytometry was employed to detect the levels of Perforin, IFN-γ and IL-10 in CD8 ^＋ cells to compare with those of the health donors the HBV DNA copies in their blood were also measured by RT-PCR to analyze the relationship with Perforin, IFN-3, and IL-10 in CD8^＋ cells. Results In patients with chronic hepatitis B, the levels of Perforin, IFN-γand IL-10 in CD8 ^＋ cells were （5.30 ± 2.62） %, （4.05 ± 2.25 ） % and （0.77 ± 0.50） %, respectively, which were statistically lower than health donors（the t values were 4.50, 4.56 and 4.20 respectively; P 〈0.01 ） ; Of 26 cases of chronic hepatitis B patients with HBeAg-positive Perforin and IFN-γin CD8 ^＋ T cells were （4.54 ± 1.93 ） % and （3.32 ± 1.59） %, respectively, significantly lower than those of the 24 chronic hepatitis B patients with HBeAg-negative （ the t values were 2.22 and 2.54, respectively ; P 〈 0. 05 ） ; And the levels of Perforin, IFN-γhad a negative relation with the HBV DNA copies （ the coefficient correlations - 0. 539 and - 0.340 ; P 〈 0.01 and P 〈 0.05）. Conclusion In patients with chronic hepatitis B, the reduced levels of Perforin, IFN-γ and IL-10 may be related with the long-term existence of HBV, and the protracted course of disease.

关 键 词：慢性乙型肝炎 T淋巴细胞 穿孔素 

分 类 号：R51[医药卫生—内科学]