解脲脲原体Parvo和T960生物群msr基因的检测  

Detection for msr gene in biovar Parvo and biovar T960 of Ureaplasma urealyticum

作　　者：叶庭路[1] 陆春[1] 陈传杰[1] 朱国兴[1] 马寒[1] 卢荣标[1] 赖维[1] 

机构地区：[1]中山大学附属第三医院皮肤性病科,广州510630

出　　处：《中华微生物学和免疫学杂志》2010年第4期377-381,共5页Chinese Journal of Microbiology and Immunology

基　　金：广东省自然科学基金（815100890100H0108）;广东省科学计划项目基金（2007B030502013）

摘　　要：目的检测解脲脲原体（Uu）是否携带介导对红霉素耐药的msr基因，并分析其在Uu两生物群间分布的差异。方法采用微量肉汤稀释法测定72株洮临床株对红霉素的体外耐性，PCR检测msrA、msrB、marC、msrD基因，并对Uu进行PCR分群。结果72株Uu的最低抑菌浓度（MIC）范围是≤0．125μg／ml～≥128μg／ml，MIC50为32μg／ml，MIC90≥128μg/ml。分群结果示Parvo生物群51株，占70．83％，1、960生物群21株，占29．17％。共检测到msrD基因的Uu24株，msrB基因12株，msrA基因1株，没有发现Uu菌株携带msrC基因。5株Uu同时检测到msrB和msrD基因，1株池同时检测到msrA、msrB和msrD基因。以MIC≥8μg／ml为耐药判定值时，两生物群对红霉素耐药性无显著差异，msrB基因主要分布在T960生物群。结论池临床菌株携带对大环内酯类耐药的msr基因（包括msrA、msrB、msrD），msrB基因主要分布在T960生物群。Objective To detect the msr gene which confers resistance to erythromycin, and analyze its distributing difference between the two biovars of Ureaplasma urealyticum. Methods Broth dilution method was used to determine the minimum inhibitory concentrations （MIC） to erythromycin among 72 U. urealyticum clinical isolates. The msrA, msrB, msrC and msrD genes detection and biotyping of U. urealyticum were conducted using PCR. Results The MICs of 72 U. urealyticum isolates to erythromycin ranged from ≤0. 125 μg/ml to ≥ 128 μg/ml. MIC50 was 32 μg/ml and MIC90 was ≥ 128 μg/ml. Biotyping showed that biovar Parvo had 51 strains （51/72, 70.83% ） and biovar T960 had 21 （21/72, 29.17% ） strains. The msrA, msrB, msrC and msrD genes were obtained in 1, 12, 0 and 24 strains, respectively, with five strains carrying the msrB and msrD genes, and one strain carrying the msrA, msrB and msrD genes. There was no resistance difference to erythromycin between the two biovars when the MIC ≥8 μg/ml was considered resistance to eryt hromyein. But the msrB gene was predominantly detected in biovar 3960. Condusion U. urealyticum clinical isolates harbour the rnsrA, msrB and msrD genes, and the predominantly detected msrB gene is of biovar T960.

关 键 词：解脲脲原体 红霉素 抗生素敏感试验 msr基因 

分 类 号：R446.5[医药卫生—诊断学]