苹果两个新S-RNase基因克隆与46个品种S基因型的PCR分析  

Characterization of Two Novel S-RNase Genes and PCR Analyzing of S-genotypes of 46 Cultivars in Malus domestica Borkh.

作　　者：龙慎山[1] 李茂福[1] 韩振海[1] 张冰冰[2] 王昆[3] 李天忠[1] 

机构地区：[1]中国农业大学果树细胞与分子育种实验室,北京100193 [2]吉林省农业科学院果树研究所,公主岭136100 [3]中国农业科学院果树研究所,兴城125100

出　　处：《农业生物技术学报》2010年第2期265-271,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基因资助项目(30671443);国家科技支撑计划项目(2007BAD3602)共同资助

摘　　要：蔷薇科苹果是一个典型的配子体型自交不亲和性果树树种,确定苹果品种的S基因型对于合理配置授粉树和杂交育种亲和性亲本选择具有重要意义。研究从苹果(Malus domestica)品种达尔文和金沙依拉姆中克隆得到了两个新S-RNase基因,分别命名为S53-RNase和S54-RNase,它们在GenBank上的登录号分别为FJ602074和FJ602075,并对46个苹果品种基因组DNA进行S等位基因特异性PCR扩增,鉴定了其S基因型。Apple（Malus domestica Borkh） is a typical Rosaceae species that exhibits gametophytic self-incompatibility（GSI）.Identifying the S-genotypes of apple cultivars have the practical significance for selecting appropriate pollinated varieties and incompatibility parents for hybridization breeding.In this investigation two novel genes named S53-and S54-RNase respectively were cloned from apple cultivars Darling and Jinshayilamu.They were deposited in GenBank under the accession numbers of FJ602074 and FJ602075, respectively.Furthermore, S-allelespecific PCR were conducted using genomic DNA extracted from 46 apple cultivars as templates and S-genotypes of these cultivars were identified.

关 键 词：苹果 自交不亲和性 S-RNase基因克隆 S-基因型鉴定 

分 类 号：S718.46[农业科学—林学]