油茶长林系列优良无性系的SRAP分子鉴别及遗传分析  

Identification and Genetic Analysis of Camellia oleifera Changlin Series Superior Clones by SRAP Molecular Marker

作　　者：林萍[1] 姚小华[1] 王开良[1] 郑婷婷[1] 滕建华 

机构地区：[1]中国林科院亚热带林业研究所,富阳311400 [2]浙江省金华市婺城区东方红林场,金华321025

出　　处：《农业生物技术学报》2010年第2期272-279,共8页Journal of Agricultural Biotechnology

基　　金：十一五国家科技支撑计划项目(2006BAD01A1706);十一五国家科技支撑计划项目(2009BADB1B01);中国林科院公益性科研基金专项(CAFYBB2008005);中国林科院亚林所公益性科研基金专项(RISF6804)共同资助

摘　　要：油茶(Camellia oleifera)良种鉴别是解决目前生产上种苗混乱的重要途径。采用SRAP方法,对油茶长林系列12个优良无性系进行分析。筛选出15对多态性高的引物,共扩增得到423个位点,多态性位点达到93.14%,品种特异性条带达50条,其中有13对引物能够独立鉴别所有供试材料。为了提高鉴别效率,选择多态性高的24个位点建立了所有供试材料的DNA指纹图谱,实现了供试材料的有效鉴别。遗传距离和聚类分析表明,长林系列无性系间存在很大的遗传差异,遗传距离的变化范围是0.5022～0.8163,取GD=0.35时,可将长林系列12个无性系分为3大类群。这为油茶良种选育提供了理论基础和依据。Cultivars identification is an important method to resolve seedling confused.SRAP were used to detect the genetic diversity of 12 Changlin series superior clones.15 primer pairs were screened out and 423 SRAP sites were amplified from these clones, of which 394 alleles were polymorphic loci and 50 markers were unique fragments.There were 13 primer pairs which could identify all clones respectively.In order to improve the identification efficiency, 24 loci were selected to establish the DNA finger print of the 12 clones.Genetic distance and cluster analysis were done, and the results showed that there was a great genetic diversity among the clones.The genetic distance（GD） was from 0.502 2 to 0.816 3, and in the level of GD 0.35, the 12 Changlin series superior clones can be divided 3 groups.All of these provide the rationale for Camellia oleifera breeding.

关 键 词：油茶 优良无性系 SRAP DNA指纹图谱 

分 类 号：S792.11[农业科学—林木遗传育种]