籽鹅卵巢组织全长cDNA文库的构建及部分克隆序列分析  

Construction of the Full-length cDNA Library and Analysis in Part of ESTs in Zi Goose Ovary

作　　者：董重阳[1] 康波[2] 贾晓剑 杨焕民[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]四川农业大学动物科技学院,雅安625014 [3]黑龙江省青年农场畜牧科,哈尔滨150050

出　　处：《农业生物技术学报》2010年第2期389-393,共5页Journal of Agricultural Biotechnology

基　　金：黑龙江省科技厅攻关项目(No.GB06B204-4)资助

摘　　要：为克隆鹅产蛋性能相关基因,以籽鹅(Anseranser)卵巢组织为实验材料,以λTriplEx2为载体,用SMART(switchingmechanism at 5'end of RNA transcript)方法构建了籽鹅卵巢组织的全长cDNA文库。结果表明,该文库初始滴度为3.1×106pfu/mL,重组率为94%,插入片段长度集中在0.5～2kb。随机挑取120个克隆测序,测序成功110个,分别对应于81个cDNA片段,与BLASTn进行序列比对,其中有70个为已公布的EST,其中大部分与原鸡或其它物种具有较高的同源性,而11个cDNA与其它物种之间同源性低,可能是未知功能基因。In order to clone the full-length of gene related to egg laying performance, the experiment was conducted to constructed the full-length cDNA library of the ovary in the Zi geese（Anser anser） by SMART（switching mechanism at 5＇ end of RNA transcript） by λTriplEx2 as vector.The results showed that the titration of the cDNA library was 3.1×10^6 pfu/mL, the rate of recombination was 94%, and the fragment length of inserted DNA ranged mainly from 0.5 kb to 2 kb.One hundred and tenty clones were chosen at random for sequencing from the cDNA library, and 110 clones were sequenced successfully, and after BLASTn analysis of cDNAs, there possible function were predict and 70 clones were known ESTs, in which most clones were high homology with gallus and other species;11 clones were novel ESTs.

关 键 词：籽鹅 卵巢 CDNA文库 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]