大孔树脂分离纯化持久霉素  

Separation and Purification of Enramycin by Macroporous Resins

作　　者：王丹丹[1] 杨文革[1] 胡永红[1] 万文倩[1] 

机构地区：[1]南京工业大学生物与制药工程学院

出　　处：《食品与发酵工业》2010年第4期194-197,共4页Food and Fermentation Industries

基　　金：江苏省科技支撑计划(农业)项目(BE2008399);江苏省高校自然科学研究计划项目(08KJA530002)

摘　　要：以吸附量、解吸率、洗脱流速等为考察指标,确定持久霉素的最佳纯化工艺为:Ab-8型树脂具有最佳的吸附及洗脱效果,在树脂柱中静止吸附的时间为0.5h,上柱液质量浓度为13.03mg/mL,最佳上样量为20mL,洗脱流速为5mL/min,用700mL的70%溶剂A与30%溶剂B去除杂质,得到纯度较高的持久霉素,经过葡聚糖凝胶纯化后的试样中,持久霉素的相对含量可达到95%以上。Macroporous resin was selected for the separation of Enramycin. Ab-8 resin was the best for its highest adsorption and desorption properties. The optimum purification conditions were as follows： adsorption time 0. 5 h, the concentration of adsorption liquid 13.03 mg/mL, adsorption volume 20 mL, elution flow rate 5 mL/min. 700 mL 70% solvent A and 30% solvent B was used to remove impurities. After being purified by the sephadex, the purity of enramycin was up to 95%.

关 键 词：持久霉素 大孔树脂 静态吸附 动态吸附 分离纯化 

分 类 号：TQ465[化学工程—制药化工]