浙江西兰花花球头腐病的病原鉴定  

Identification of the pathogen of on broccoli head rot in Zhejiang Province

作　　者：王国良[1] 李斌[2] 唐乔梅[2] 刘宝平[2] 张国庆[2] 苏婷[2] 

机构地区：[1]浙江万里学院生命科学系,宁波315100 [2]浙江大学生物技术研究所农业部病虫分子生物学开放重点实验室,杭州310029

出　　处：《植物保护学报》2010年第2期123-127,共5页Journal of Plant Protection

基　　金：中央高校基本科研业务费专项资金;浙江省自然科学基金(Y3090150);国家自然科学基金(30600475)

摘　　要：为明确近年来浙江省宁波市部分地区发生的西兰花花球头腐病的病原,通过田间症状观察、致病性测定、Biolog和脂肪酸(fatty acid methyl ester,FAME)分析将分离到的病原菌鉴定为荧光假单胞菌Pseudomonas fluorescens。利用16S rDNA和核糖体DNA内转录间隔区(internal transcribed spacer,ITS)序列构建的系统发育树也都显示分离的西兰花菌株与假单胞菌聚合成群,其中与荧光假单胞菌的亲缘关系最近,序列同源性分别为99%和98%。表明浙江省西兰花花球上发生的细菌性头腐病由荧光假单胞菌引起。In order to elucidate the causal agent of broccoli head rot in Ningbo, Zhejiang Province, the causal bacterium was isolated and characterized with the pathogenicity test, Biolog and FAME （fatty acid methyl ester） test. The results indicated that the causal agent belong to Pseudomonas fluorescens. The Ningbo strains were most closely related to P. fluorescens in the phylogenetic trees constructed based on the 16S rDNA gene and 16S-23S rDNA internal transcribed spacer （ITS） gene of the Ningbo strains and related species, respectively, while the 16S rDNA and ITS gene sequences of the Ningbo strains had 99% and 98% similarity with that of P.fluorescens, respectively. Overall, this result showed that P.fluorescens is the causal agent of broccoli head rot.

关 键 词：西兰花 头腐病 病原鉴定 荧光假单胞菌 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]