多药耐药铜绿假单胞菌MexAB-OprM主动外排系统的耐药作用  

Contribution of MexAB-OprM Active Efflux System in Multidrug-resistant Pseudomonas aeruginosa

作　　者：魏志华[1] 沈继录[1] 徐元宏[1] 

机构地区：[1]安徽医科大学第一附属医院检验科,安徽合肥230022

出　　处：《中华医院感染学杂志》2010年第9期1205-1207,共3页Chinese Journal of Nosocomiology

基　　金：安徽省自然科学基金资助(09020103002)

摘　　要：目的探讨MexAB-OprM主动外排系统(外排泵)在多药耐药铜绿假单胞菌耐药机制中的作用。方法挑选临床分离的铜绿假单胞菌多药耐药菌株26株、敏感菌株15株及标准质控菌株(ATCC27853),用RT-PCR扩增其内膜转运蛋白mexB的结构基因mexB和内参照基因16S rRNA片段,根据mexB与16S rRNA扩增产物的电泳扫描比值,分析mexB mRNA的表达水平,判断MexAB-OprM的表达情况。结果 26株多药耐药菌株对14种常用抗菌药物全部耐药,耐药率为100%,15株敏感菌株对14种常用抗菌药物全部敏感;标准质控菌株、所有的敏感菌株和多药耐药菌株均检测出mexB mRNA基因;耐药菌株的mexB mRNA水平(2.41±1.10)显著高于敏感菌株(1.47±0.83)(P<0.01)。结论 MexAB-OprM主动外排系统表达水平的增高与铜绿假单胞菌多药耐药的形成密切相关。OBJECTIVE To investigate the effect of MexAB-OprM active efflux system in multidrug-resistant Pseudomonas aeruginosa.METHODS Totally 26 strains of multidrug-resistant P.aeruginosa,15 strains of susceptible P.aeruginosa and ATCC27853.were selected the levels of mexB mRNA of 15 standard susceptible strains and 26 multidrug-resistant strains were detected by RT-PCR,and the 16S rRNA gene was also amplified as control.The levels of mexB mRNA were analyzed according to the ratio of electrophoresis scan results of their PCR products and the 16S rRNA′s.RESULTS The drug-resistant rates of 26 strains of multidrug-resistant P.aeruginosa against 14 kinds of antibiotics were 100%.The mexB mRNA were examined in all strains.The levels of mexB mRNA in multidrug-resistant strains（2.41±1.10） were significantly higher than that of standard susceptible strains（1.47±0.83）（P〈0.01）.CONCLUSIONS The overexpression of MexAB-OprM active efflux system is associated with multidrug resistance of P.aeruginosa.

关 键 词：铜绿假单胞菌 外排泵 耐药性 

分 类 号：R378.99[医药卫生—病原生物学]