多药耐药肺炎克雷伯菌可移动遗传元件研究  

Detection of Mobile Genetic Elements in Multidrug-resistant Klebsiella pneumoniae

作　　者：凌月明[1] 陈金玉[1] 郭勤华[1] 蔡媛媛[1] 赵婉婷[1] 赵桂梅[1] 

机构地区：[1]解放军第180医院检验病理科,福建泉州362000

出　　处：《中华医院感染学杂志》2010年第9期1208-1211,共4页Chinese Journal of Nosocomiology

摘　　要：目的了解多药耐药肺炎克雷伯菌(MDR-KPN)耐药基因载体-可遗传元件分布状况。方法运用PCR法对20株MDR-KPN进行了接合性质粒遗传标记(traAt、rbC)、插入序列遗传标记(IS1133I、SEcp1)、转座子遗传标记(merAt、npUt、np513)、整合子遗传标记(intⅠ1i、ntⅠ2i、ntⅠ3)等4类可移动遗传元件检测。结果 trbC、ISEcp1、merAt、npUt、np513i、ntⅠ1检出阳性率分别为:55.0%、65.0%、25.0%、20.0%、50.0%、65.0%,其余4种基因均未检测到可移动性元件;20株菌株每株至少检出1种可移动遗传元件遗传标记,其中在MDR-KPN中检出ISEcp1、merA、tnpUt、rbC基因属国内首次。结论多药耐药肺炎克雷伯菌易检出各类可移动遗传元件,与医院多耐药肺炎克雷伯菌耐药机制相关的可移动遗传元件主要有trbCI、SEcp1、merAt、npUt、np513i、ntⅠ1。OBJECTIVE To investigate the distribution of the drug-resistant genetic carrier-mobile genetic elements in multidrug-resistant Klebsiella pneumoniae.METHODS Four kinds of mobile genetic elements,such as conjugal plasmid genetic marks（traA and trbC）,cut-in sequence genetic marks（IS1133 and ISEcp1）,transposon genetic marks（merA,tnpU and tnp513） and integron genetic marks（intⅠ1,intⅠ2 and intⅠ3） in 20 cluster MDR-KPN strains were assayed by polymerase chain reaction（PCR）.RESULTS Among 20 strains of MDR-KPN,the positive rates of trbC,ISEcp1,merA,tnpU,tnp513,intⅠ1 were 55.0%,65.0%,25.0%,20.0%,50.0% and 65.0%,respectively.and the other 4 genes were negative.At least one mobile genetic element was found in each MDR-KPN strain.It was first reported that ISEcp1,merA,tnpU and trbC were found positive in MDR-KPN in china.CONCLUSIONS Mobile genetic elements can be mostly found positive in MDR-KPN.trbC,ISEcp1,merA,tnpU,tnp513,and intⅠ1 may be the main mobile genetic elements which induce multi-resistant mechanism of MDR-KPN in our hospital.

关 键 词：肺炎克雷伯菌 多药耐药 可移动遗传元件 

分 类 号：R378.99[医药卫生—病原生物学]