川芎治疗偏头痛效应组分的体内移行研究  

In Vivo Transmigration of Anti-Migrainous Compounds from Ligusticum chuanxiong Hort

作　　者：袁莹[1] 林晓[2,1] 冯怡[1] 徐德生[1,3] 汪益函[2] 

机构地区：[1]上海中医药大学中药现代制剂技术教育部工程研究中心,上海201203 [2]上海中医药大学中药学院,上海201203 [3]上海曙光医院,上海200021

出　　处：《中国药学杂志》2010年第9期694-697,共4页Chinese Pharmaceutical Journal

基　　金：上海市教育委员会科研创新项目(09YZ117);上海市教育委员会重点学科资助项目(J50302)

摘　　要：目的借鉴血清药物化学的方法,探讨川芎效应组分在血液及靶组织脑脊液中的移行成分(即药物以原型进入到体液的化学成分),从而为快速、有效地确定川芎效应组分的物质基础,进一步建立其完善的质量标准奠定基础。方法通过HPLC指纹图谱和紫外光谱分析,比较空白血清、含药血清,空白脑脊液、含药脑脊液成分异同,确定川芎效应组分的血中移行成分与脑脊液移行成分,并采用HPLC-DAD-MS/MSn分析技术推测移行成分的化学结构。结果阿魏酸、洋川芎内酯Ⅰ为川芎效应组分的血清移行成分,洋川芎内酯Ⅰ为其脑脊液移行成分,即洋川芎内酯Ⅰ为川芎效应组分即入血又入脑的化学成分。结论洋川芎内酯Ⅰ很可能是川芎效应组分治疗偏头痛最重要的生物活性成分。OBJECTIVE To study the absorbed constituents of the Chuanxiong active parts （CXAP） for the treatment of migraine following oral administration.METHODS The compound peaks of blank serum,drug-contained serum and cerebrospinal fluid were compared by HPLC fingerprints and UV spectrums.The absorbed ingredients of CXAP were structurally identified by HPLC-DAD-MS/MSn.RESULTS Following oral administration of CXAP,ferulic acid and senkyunolide I were found in serum.However,only the latter was detectable in the cerebrospinal fluid.CONCLUSION Senkyunolide I may be the most important bioactive component of Chuanxiong for the treatment of migraine.

关 键 词：川芎 效应组分 移行成分 物质基础 

分 类 号：R917.1[医药卫生—药物分析学]