小口径组织工程血管体外构建的研究  

Construction of small-caliber tissue engineered blood vessel in vitro

作　　者：李春民[1] 董建德[2] 谷涌泉[3] 邱荣鑫[4] 冯增国[4] 孟艳[3] 陈晓波[1] 汪忠镐[3] 

机构地区：[1]首都医科大学附属复兴医院血管外科,北京100038 [2]北京电力总医院胸心血管外科 [3]首都医科大学宣武医院血管外科 [4]北京理工大学材料科学与工程学院

出　　处：《中华实验外科杂志》2010年第5期561-563,682,共4页Chinese Journal of Experimental Surgery

基　　金：国家高技术研究发展计划863计划资助项目（2006AA0-2A134）;国家自然科学基金资助项目（30640078）

摘　　要：目的探讨间充质干细胞体外构建组织工程血管的可行性。方法将体外培养扩增的犬骨髓间充质干细胞（MSCs）定向分化为平滑肌样细胞和内皮样细胞，接种于ε-己内酯／L-丙交酯（PCLA）支架上，将其置于生物反应器内，在搏动性力学（100±20／55±20）mmHg（1mmHg=0．133kPa）刺激条件下培养。3d后行血管组织学检测。结果 血管支架拉伸强度6．1MPa；骨髓间充质干细胞成功定向分化为平滑肌样细胞和内皮样细胞；血管腔内表面完全为细胞覆盖，表面的细胞沿液体流动的方向分布；种植的部分细胞已经渗透人血管壁内。结论骨髓间充质干细胞可作为种子细胞，与PCLA支架在生物反应器内构建组织工程血管。Objective To investigate the feasibility of constructing small-caliber tissue engineered blood vessels in vitro. Methods Canine mesenehymal stem cells （MSCs） were cultured and differentiated to endothelial cells （ECs） and smooth muscular cells （SMCs） in vitro. The SMCs and ECs were seeded on the lumen of the PCLA scaffold. The scaffold was connected to a pulsatile flow chamber in bioreactor. After its culturing in a bioreaetor with mechanical stimulation （ 100±20/55±20） nun Hg （ 1 mm Hg =0. 133 kPa） for 3 days, the scaffold was observed under the scanning electron microscopy and by using immunofluorescence staining. Results The tensile strength of scaffold was 6. 1 MPa. The MSCs were differentiated to ECs and SMCs. A confluent cellular monolayer covering the inner surface of the scaffold was found. Cells were elongated and aligned in the direction of the blood flow. The seeding cells were infiltrated into the interstitia of the PCLA scaffold. Conclusion MSCs as seeding cells and PCLA as scaffold are able to construct a tissue engineering vessel in a bioreactor.

关 键 词：间充质干细胞 组织工程 血管 

分 类 号：R318.08[医药卫生—生物医学工程]