alpha-1抗胰蛋白酶在升主动脉夹层及瘤样扩张血管组织中的表达  

The expression of alpha-1 antitrypsin in ascending aortic dissection and aneurysm tissues

作　　者：陆树洋[1] 王春生[1] 宋凯[1] 杨守国[1] 潘荪[1] 洪涛[1] 

机构地区：[1]上海市心血管病研究所复旦大学附属中山医院心外科,200032

出　　处：《中华实验外科杂志》2010年第5期564-566,682,F0003,共5页Chinese Journal of Experimental Surgery

基　　金：上海市重点学科建设资助项目（BI16）;上海市教育委员会曙光计划资助项目（03SG05）

摘　　要：目的观察正常升主动脉、升主动脉瘤样扩张及StanfordA型夹层动脉瘤（TAAD）患者升主动脉血管组织中alpha-1抗胰蛋白酶表达的差异，探讨alpha．1抗胰蛋白酶对于维持升主动脉血管结构完整性中的作用。方法收集28例升主动脉血管组织标本，TAAD14例，升主动脉瘤样扩张7例，心脏移植供体心脏升主动脉7例。采用组织学、逆转录一聚合酶链反应（RT-PCR）法及Westernblot法对标本组织中的alpha-1抗胰蛋白酶进行检测分析。结果在基因水平上，alpha-1抗胰蛋白酶在升主动脉瘤样扩张中表达最高，正常血管组织中表达次之，TAAD患者升主动脉中表达最低（2．192±0．133、1．213±0．156、0．672±0．101，P〈0．05）。在蛋白水平上，alpha-1抗胰蛋白酶在升主动脉瘤样扩张中表达明显升高，TAAD患者升主动脉中表达次之，正常血管组织中最低（0．285±0．010、0．153±0．011、0．102±0．010，P〈0．05）。结论alpha-1抗胰蛋白酶在正常人、升主动脉瘤样扩张及TAAD患者升主动脉血管组织均有表达，表达差异有统计学意义。alpha-1抗胰蛋白酶对于维持升主动脉血管结构的完整性具有潜在的保护作用。Objective To investigate whether alpha-1 antitrypsin is present in normal human aorta, ascending aortic aneurysm and stanford A type aortic dissection （TAAD） tissues and whether there are some expression differences in alpha-1 antitrypsin levels among them, and discuss its potential protective effects on aortic wall structure. Methods Histological method, semi-quantified reverse transcription poly- merase chain reaction （RT-PCR） and Western blotting were used to test whether alpha-1 antitrypsin was present in normal human aorta, ascending aortic aneurysm and TAAD tissues. TAAD tissues in 14 cases, ascending aortic ancurysm tissues in 7 cases and normal ascending aorta tissues in 7 cases were harvested. Results alpha-1 antitrypsin expression was strongly increased in TAAD and ascending aortic aneurysm tis- sues as compared with normal aorta （0. 285 ±0. 010, 0. 153 ±0. 011,0. 102 ±0. 010,P 〈0. 05）. alpha-1 antitrypsin was increased more significantly in ascending aortic aneurysm than in TAAD （0. 285± 0. 010 vs 0. 153±0. 011 ,P 〈0. 05）. RT-PCR revealed that there was endogenous alpha-1 antitrypsin produced in aortic wall, and mRNA expression levels of them were highest in ascending aortic aneurym and lowest in TAAD （2. 192±0. 133, 1. 213 ±0. 156, 0. 672 ±0. 101 ,P 〈0. 05）. Conclusion alpha-1 antitrypsin is present in normal human aorta, ascending aortic aneurysm and TAAD tissues, and there are differential expression differences among them. alpha-1 antitrypsin presents a potential pathway for protection of aortic wall structure.

关 键 词：抗胰蛋白酶 动脉瘤 升主动脉 

分 类 号：R654.3[医药卫生—外科学]