15-脱氧-△^12,14，-前列腺素J2对兔耳增生性瘢痕Ⅰ型胶原表达的影响  

Effects of 15-deoxy-△^12,14,-prostagliandxin J2 on the expression of type I collagen in hypertrophic scar of rabbit ears

作　　者：潘姝[1] 李叶扬[1] 方力[2] 祁少海[3] 舒斌[3] 梁佩红[1] 戴丽冰[1] 李建平[2] 张琰[1] 李罡[1] 

机构地区：[1]暨南大学第四附属医院广州市红十字会医院烧伤整形科,广州510220 [2]暨南大学第四附属医院广州市红十字会医院病理科,广州510220 [3]中山大学第一附属医院烧伤科

出　　处：《中华实验外科杂志》2010年第5期577-579,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30600642）;广州市医药卫生科技重点项目（2006-ZDi-08）

摘　　要：目的观察过氧化物酶体增殖物激活受体γ（PPARγ）的配体15-脱氧-△^12,14，-前列腺素J2（15d-PGJ2）对兔耳增生性瘢痕Ⅰ型胶原表达的影响，探讨15d—PGJ2防治增生性瘢痕的可行性。方法选取新西兰大白兔18只，在兔耳腹侧面制作2cm×3cm全层皮肤缺损创面，每耳2个，共计72个，建立兔耳增生性瘢痕动物模型，随机分组，分别用15d—PGJ2及生理盐水行瘢痕内注射，1次／d，共7次。停药后第14、21天两组同时取材；每组每次切取18个组织块。应用免疫组织化学、荧光定量聚合酶链反应（PCR）及Westernblot检测Ⅰ型胶原的表达。结果与对照组比较，15d．PGJ2注射后瘢痕体积缩小，变软变平，色泽轻度变浅。Ⅰ型胶原主要分布于真皮的细胞间质、成纤维细胞胞质中，血管壁上亦见阳性信号，在各个时间点15d—PGJ2组Ⅰ型胶原mRNA和蛋白的表达均较对照组低，且差异有统计学意义（P〈0．05）。结论PPAR-1的配体15d—PGJ2可降低瘢痕内Ⅰ型胶原的含量，引起瘢痕萎缩，从而防治瘢痕。Objective To investigate the effect of 15d-PGJ2 on the expression of type Ⅰ collagen in hypertrophic scar of rabbit ears, and the possibility of hypertrophic scar treated by 15d-PGJ2. Methods Eighteen New Zealand white rabbits were used to establish the hypertrophic scar models on the rabbit ears. The wounds were created as follows ： 2 cm × 3 cm wounds with total skin loses on the ventral side, 2 wounds for each ear, total 72 wounds. The wounds were randomly divided into the 15d-PGJ2 treatment group and NS control group. A total of 20μl 15d-PGJ2 or NS was injected into the ear scar once a day for 7 days. At the 14th and 21st day after the injection, 18 scars in each group were harvested. The expression levels of type Ⅰ collagen was detected by immunohistochemistry, fluorescence quantitative polymerase chain reaction （PCR） and Western blotting. Results Compared with the NS control group, the 15d-PGJ2-treated scars appeared to be smaller, softer, flatter and lighter in color. The expression of type Ⅰ collagen was mainly distributed in matrix, fibroblast cytoplasma and the vascular wall. The mRNA and protein levels of type Ⅰ collagen were significantly decreased in the 15d-PGJ2-treated group as compared with those in the NS control group at different time points （P 〈 0. 05）. Conclusion 15d-PGJ2, the ligand of PPAR-γ, can reduce the expression of type Ⅰ collagen in hypertrophic scar of rabbit ears and plays an important role in the prevention and treatment of hypertrophic scar. It offers a new way for treating the hypertrophic scar clinically.

关 键 词：增生性瘢痕 PPAR-Γ 胶原 

分 类 号：R622[医药卫生—整形外科]