淫羊藿苷对去卵巢快速老化小鼠(SAMP8)学习记忆及皮层氧化应激的影响  

Effect of Icariin on learning and memory,cortex oxidative stress in ovarietomized senescence-accelerated prone/8 female mice

作　　者：刘东华[1] 吴满刚[1] 毕明刚[2] 杜冠华[3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076 [2]中国医学科学院药用植物研究所,北京100193 [3]中国医学科学院药物研究所,北京100050

出　　处：《军医进修学院学报》2010年第6期580-583,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30772750);"重大新药创制"科技重大专项(2009ZX09301-003)~~

摘　　要：目的研究淫羊藿苷(ICA)对去卵巢雌性快速老化模型小鼠(SAMP8)学习记忆能力及抗氧化能力的影响。方法本实验以10月龄去卵巢雌性SAMP8小鼠为模型,以同月龄抗快速老化小鼠(SAMR1)为正常对照,以SAMP8假手术作为对照。实验共分为SAMR1组、SAMP8假手术组、SAMP8手术组、多奈哌齐组、己烯雌酚组、ICA75mg/kg组、ICA150mg/kg组7组。灌胃给药8周,通过Morris水迷宫检验ICA对SAMR1和SAMP8学习记忆能力的影响;测定大脑皮层中NO、MDA、SOD、GSH-Px活性。结果ICA150mg/kg、75mg/kg可明显改善OVX SAMP8定位航行潜伏期(P<0.05),提高空间探索能力,平台象限停留时间为ICA150mg/kg45.88±2.98s,ICA75mg/kg40.26±3.66s,OVX SAMP817.13±3.73s(P<0.05);可明显提高大脑皮层内SOD及GSH-Px活性(P<0.05),显著降低MDA及NO的水平(P<0.05)。结论ICA可以改善OVX SAMP8的学习记忆能力,可显著提高OVX SAMP8抗氧化能力。Objective To investigate the effects of icariin（ICA）on learning and memory,and antioxidative ability of ovariectomized senescence accelerated prone/8 female mice.Methods Female mice with ovariectomized senescence-accelerated prone/8（SAP8）at the age of 10 months were divided into model group,normal group and sham operation group.Experimental group was further divided into senescence-accelerated resistant mouse1（SAMR1）group,sham operation group,SAP8 group,donepezil group,stilbestrol group,ICA75mg/kg group and ICA150mg/kg group.After 8 weeks of intragastric administration of ICA,Morris water maze test was used to test the effects of ICA on learning and memory in SAMR1 and SAP8 groups.NO and MDA level,and SOD and GSH-Px activity in cerebral cortex were measured.Results ICA（150mg/kg or 75mg/kg）could significantly improve the latency on place navigation of OVX SAP8,space exploration,and residence times on quadrant of platform,reduce MDA and NO level,and increase SOD and GSH-Px activity in cerebral cortex（P0.05）.Conclusion ICA can improve learning and memory of ovariectomized SAP8 female mice.

关 键 词：淫羊藿苷 快速老化小鼠 学习 记忆 抗氧化 

分 类 号：R285.5[医药卫生—中药学]