离乳1周SPF级与普通级新西兰兔血液学参数比较  

Comparison of Hematological Parameters of SPF and Conventional New Zealand Rabbits in Weaning 7 Day

作　　者：王刚[1] 张富发[1] 刘科[1] 赵伟健[1] 刘盛来[1] 饶子亮[1] 邝少松[1] 

机构地区：[1]广东省医学实验动物中心,广州510180

出　　处：《实验动物科学》2010年第2期26-29,共4页Laboratory Animal Science

基　　金：广东省科技计划资助项目(2007A060305012)

摘　　要：目的测定离乳1周龄SPF级和普通级新西兰兔的血液生理生化参数,并进行比对研究。方法选取离乳1周龄健康的SPF级和普通级新西兰兔各20只,均雌雄各半;全部动物耳缘静脉取血,运用血液分析仪、全自动生化分析仪,对所采血液进行生理生化指标测定;将测定数据输入计算机,用SPSS软件统计分析,计算均数和标准差(x珋±s),并做t检验。结果离乳1周龄SPF级雌性与雄性兔相比较,白细胞数、总蛋白指标差异极显著(P<0.01),红细胞、葡萄糖等6项指标差异显著(P<0.05)。普通级雌兔和雄兔在平均血红蛋白浓度、淋巴细胞比率、总胆红素等3项指标差异显著(P<0.05)。SPF级雄兔和普通级雄兔比较,白细胞、中性粒细胞、碱性磷酸酶等20项指标差异极显著(P<0.01),红细胞、嗜碱性粒细胞等5项指标差异显著(P<0.05)。SPF级雌兔和普通级雌兔相比较、在白细胞数、中性粒细胞、碱性磷酸酶等9项指标差异极显著(P<0.01),在平均血红蛋白浓度、嗜酸性粒细胞等4项指标差异显著(P<0.05),其余差异不显著(P>0.05)。结论同等级新西兰兔血液生理生化参数差异较小,不同微生物控制等级对新西兰兔血液生理生化参数有显著影响。Objective To determine and compare the hematological parameters of SPF and conventional New Zealand rabbits in weaning 7 day.Methods Hematological parameters of the SPF and conventional New Zealand rabbits were detected using an automatic hematology analyzer and clinical chemistry analyzer.Results For SPF rabbits,the WBC and Tp have had very significant difference between female and male（P 0.01）.The 6 indexes as RBC,GLU have significant differences（P 0.05）.For conventional rabbits,the only 3 indexes as MCHC,LY%,TBIL have significant differences（P 0.05）.Comparing SPF and conventional male rabbits,the 20 indexes as WBC,NE,ALP have very significant differences（P 0.01）,the 5 indexes as RBC,BA have significant differences（P 0.05）.Comparing SPF and conventional female rabbits,the 9 indexes as WBC,NE,ALP have very significant differences（P 0.01）,the 4 indexes as MCH,EO have significant differences（P 0.05）,other indexes had not significant difference（P 0.05）.Conclusion For New Zealand rabbits,the difference of the hematological parameters in same microbiological background is smaller.The Different microbiological background significantly affects the hematological parameters in New Zealand rabbits.

关 键 词：新西兰兔 SPF级 普通级 血常规 血生化 

分 类 号：S865.1[农业科学—野生动物驯养]