参附注射液预处理对大鼠肝缺血再灌注肠黏膜屏障的保护作用  

Effect of Shenfu injection on intestinal mucosal barrier of Rats with hepatic ischemia-reperfusion

作　　者：戴维[1] 陈念平[1] 陈明[1] 缪辉来[1] 秦勇[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东省湛江524023

出　　处：《中国基层医药》2010年第9期1185-1186,共2页Chinese Journal of Primary Medicine and Pharmacy

基　　金：基金项目：广东省湛江市科技基金资助项目（2008C04008）

摘　　要：目的 探讨参附注射液（SF）对大鼠肝缺血再灌注损伤肠黏膜的保护作用。方法选用健康雄性SD大鼠90只，阻断第一肝门建立肝缺血再灌注模型，随机分为假手术组（SO组）、0．9％氯化钠注射液（NS）对照组（IR＋Ns组）和参附注射液治疗组（IR＋sF组），SO组和IR＋NS组于再灌注前用与SF注射液等量之NS注射预处理，IR＋sF组则用SF灌注前做预处理，每组30只。建立肝缺血再灌注模型后再分为6h和12h两个时相点组，每组15只。检测血浆内毒素含量、ALT、丙二醛（MDA）和统计肠系膜淋巴结细菌移位率，并用光镜观察肠组织的病理变化。结果细菌移位率、内毒素、ALT和MDA水平IR＋sF组均较1R＋Ns组低。结论参附注射液对肝缺血再灌注后的肠黏膜屏障损伤有保护作用。Objective To observe the effects of Shenfu injection on intestinal mucosal barrier of rats with hepatic ischemia-reperfusion. Method Ninety SD male rats and weight from 230 to 270g were randomly divided into three groups（30 rats in each） ,and 30 rats in each group divided again into 6h and 12h group（ 15 rats in each） ： （ 1 ） SO group received sham operation（SO） ; （2） IR ＋ NS group received so and 0.9 normal saline intravenously imediately before the ischemia reperfusion injury;（ 3 ）IR ＋ SF group received Shenfu injection intravenously imediately before the ischemia reperfusion injury ,IR ＋ NS group and IR ＋ SF group occlusion of the hepatoduodenal ligament ,and endotoxin, ALT and MDA were detected. Results The levels of ALT, MDA in IR ＋ NS group were higher than those in IR ＋ SF group,and the rate of bacterial translocation（BT） group.

关 键 词：参附注射液 再灌注损伤 细菌移位 肠黏膜 

分 类 号：R28[医药卫生—中药学]