用正交试验设计优选抗眩晕颗粒的半仿生提取工艺条件  

Orthogonal Design for Optimizing the Semi-bionic Extraction of Kangxuanyun Granules

作　　者：王洪志[1] 乔晓莉[1] 李婉晴[1] 刘勇[1] 樊华[1] 

机构地区：[1]天津市南开医院,天津300100

出　　处：《中国实验方剂学杂志》2010年第5期19-24,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市卫生局中西医结合资助课题(07018)

摘　　要：目的:以抗眩晕颗粒为例,探讨中药复方半仿生提取(semi-bionic extraction,简称SBE)的研究方法。方法:以泽泻醇B乙酸酯、川芎嗪、阿魏酸、总生物碱、大黄素和大黄酚之和、干浸膏为指标成分,用正交试验优选半仿生提取的工艺条件。结果:优选出的工艺条件为:用10%HCl调第1煎pH值为2.00,用10%NaOH分别调第2煎、第3煎pH值为7.50、8.50,溶剂用量依次为10,8,6倍,煎煮时间依次为2.0,1.5,1.5h。结论:验证试验结果表明,优选出的SBE工艺条件稳定、可行,优于原工艺条件。Objective：To investigate the method for using semi-bionic （SBE） on Kangxuanyun granules as an example. Method： Orthogonal design was used to optimize the extraction conditions of SBE with the evaluation markers such as alisol B-acetate,tetramethylpyrazine,ferulic acid,total alkaloids,the total contents of emodin and chrysophanol,dried extract. Result： The optimized SBE conditions were as follows： The pH of the three extractions were in proper order 2. 00,7. 50,8. 50; the solvent volume was 10,8,6 times; and the extraction time was 2. 0, 1. 5,1. 5 h,respectively. Conclusion： The optimum extraction conditions are stable and practicable,and surpass the original extraction conditions.

关 键 词：抗眩晕颗粒 半仿生提取 正交设计 高效液相色谱法 紫外分光光度法 综合评价 

分 类 号：R283.6[医药卫生—中药学]