薄层扫描法测定广西匙羹藤中牛弥菜醇A的含量  

Determination of Conduriol A in Guangxi Gymnema sylvestre(Retz. )Schult by TLCS

作　　者：丘琴[1,2] 甄汉深[1] 肖聪 黄小秋[1] 

机构地区：[1]广西中医学院,南宁530001 [2]成都中医药大学,成都611137 [3]广西桂林药检所,广西桂林541000

出　　处：《中国实验方剂学杂志》2010年第5期54-56,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西自然科学基金项目(桂科自0229044);广西教育厅科研项目(桂教科2001);广西中医学院重点科研项目(ZD2004010);广西高校人才小高地建设创新团队资助计划[桂教人(2005)80号]

摘　　要：目的:采用薄层扫描法测定广西匙羹藤Gymnema sylvestre(Retz.)Schult中牛弥菜醇A的含量。方法:使用硅胶H板,展开剂为苯-乙酸乙酯-甲醇(10∶3∶5),5%香草醛硫酸溶液显色,于105℃加热至斑点清晰,在200nm～800nm范围内进行扫描,检测波长538nm,参比波长700nm。结果:牛弥菜醇A的线性范围为1.025～6.150μg,平均加样回收率为98.84%,RSD=2.02%(n=6),匙羹藤中牛弥菜醇A的含量为2.08%。结论:该法简便、灵敏、准确性高,重复性好,可为匙羹藤药材的定性鉴别和含量测定提供科学依据。Objective：To determine the content of conduriol A in Guangxi Gymnema sylvestre（Retz. ）Schult by TLCS. Method：Silica H thin layer plate was used; the developer reagent was benzene：ethyl acetate：methanol （10∶ 3∶ 5）,the color reagent was 5% vanillin sulfate solution. The plate was at heating on 105 ℃ ,when the spot was clear; the detection wavelength was 538 nm and the reference wavelength was at 700 nm. Result： The conduriol A was linear in range of 1. 025 ～ 6. 150 μg; the average recovery was 98. 84% ,RSD = 2. 02% （n = 6）. The average content of conduriol A was 2. 08% in was simple,sensitive,accurate and had good reproducibility. Conclusion： The method can be used the identification and determination method of Guangxi Gymnema Sylvestre（ Retz. ） Schult and qualitatively.

关 键 词：匙羹藤 牛弥菜醇A 薄层扫描法 

分 类 号：R284.1[医药卫生—中药学]