六味地黄汤、补中益气汤、复方丹参饮对骨髓抑制小鼠保护的作用机制  

Effects of Liuwei Dihuang Decoction,Buzhong Yiqi Decoction and Compound Danshen Decoction on the Marrow-suppressed Mice

作　　者：李恩庆[1] 赵安斌[1] 曹克俭[2] 陈孝银[1] 戴汉源[1] 吴先林[1] 

机构地区：[1]暨南大学医学院,广州5106322 [2]香港大学中医药学院,香港999077

出　　处：《中国实验方剂学杂志》2010年第5期153-157,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：庞鼎元中西医学教研基金资助

摘　　要：目的:进一步研究补肾阴、补气、活血中药方对骨髓抑制小鼠保护的作用机制。方法:使用实时荧光定量RT-PCR检测小鼠脾脏促血小板生成素(thrombopoietin,TPO)mRNA、促血小板生成素受体(c-Mpl)mRNA及转录因子GATA-1mRNA表达水平。结果:六味地黄汤、补中益气汤均能明显升高骨髓抑制小鼠TPO,c-Mpl及GATA-1mRNA的表达。复方丹参饮对小鼠脾脏TPO,c-Mpl及GATA-1mRNA表达影响不明显。结论:六味地黄汤、补中益气汤均能通过升高骨髓抑制小鼠TPO,c-Mpl及GATA-1mRNA的表达水平从而促进骨髓抑制小鼠的外周血恢复。Aim：To study the protective therapeutic actions and mechanism of Liuwei Dihuang Decoction, Buzhong Yiqi Decoction and Compound Danshen Decoction on the marrow-suppressed mice. Method： The expression of thrombopoietin （ TPO ） mRNA,thrombopoietin receptor （ c-Mpl） mRNA and GATA-1 mRNA was detected by RT-PCR technique. Result： Liuwei Dihuang Decoction and Buzhong Yiqi Decoction were shown to promote the expression of TPO mRNA,c-Mpl mRNA and GATA-1 mRNA in spleen of mice. The result indicates the decoctions could accelerate the marrow cells of the marrow-suppressed mice. Although Compound Danshen Decoction did not show significant promotion for the expression of TPO mRNA,c-Mpl mRNA and GATA-1 mRNA in spleen of mice,but it also could accelerate peripheral blood cells of the marrow-suppressed mice. Conclusion： Peripheral blood cells of marrow-suppressed mice were increased significantly by Liuwei Dihuang Decoction, Buzhong Yiqi Decoction and Compound Danshen Decoction.

关 键 词：六味地黄汤 补中益气汤 复方丹参饮 骨髓抑制 促血小板生成素mRNA 促血小板生成素受体mRNA 转录因子GATA-1mRNA 

分 类 号：R285.5[医药卫生—中药学]