实时荧光定量PCR在流感病毒核酸检测中的应用  

Application of Real-time PCR in Nucleic Acid Test of Influenza Virus

作　　者：刘建荣[1] 宋淑娥[1] 何莹[1] 姬莉莉[1] 徐大鹏[1] 

机构地区：[1]北京市怀柔区疾病预防控制中心,101400

出　　处：《职业与健康》2010年第9期1000-1002,共3页Occupation and Health

摘　　要：目的通过对2009年6—11月北京怀柔地区流行性感冒(流感)病原学监测数据的分析,了解北京市怀柔区甲型H1N1型流感的流行趋势,为该地区甲型H1N1流感的防治提供科学依据。方法依据《全国流感监测实施方案》、《甲型H1N1流感病毒实验室检测技术方案》、《甲型H1N1流感监测方案(第二版)》、7500Fast型实时荧光定量PCR的SDS软件操作。结果2009年6—11月共采集流感样病例咽拭子1780件,检出流感病毒核酸阳性507件,阳性率为28.48%。阳性样本中,季节性甲3亚型92件,占18.15%;季节性甲1亚型8件,占1.58%;乙型4件,占0.79%;甲型H1N1型395件,占77.91%。结论2009年6—9月,甲型H1N1型流感的流行趋势为上升状态,但期间季节性甲3亚型仍为主要流行毒株。10—11月,甲型H1N1型流感的上升趋势达到高峰期,甲型H1N1流感病毒为流感流行主要毒株。[Objective]Through analysis on pathogen monitoring data of influenza virus in Huairou District of Beijing during June to November in 2009,to analyze the epidemic trend of Influenza A H1N1,and provide scientific evidence for control of it.[Methods]The analysis was based on ＂Implementation of National Influenza Surveillance Program＂,＂Laboratory Testing Technology solutions for Influenza A H1N1 Influenza Virus＂,＂Influenza A H1N1 Surveillance Program（2nd Edition）＂ and the SDS software of 7500 Fast real-time fluorescence quantitative PCR.[Results]Totally 1780 swab samples of influenza-like illness were collected,507 were detected influenza virus nucleic acid positive,the positive rate was 28.48%.Of 507 isolates,92（18.15%）were seasonal influenza A（H3N2）viruses,8（1.58%）were seasonal influenza A（H1N1）viruses,4（0.79%）were influenza B viruses,395（77.91%）were influenza A（H1N1）viruses.[Conclusion]The epidemic trend of influenza A H1N1 viruses was on the rise from June to September in 2009,seasonal influenza A（H3N2）strain was the main virus.From October to November,epidemic trend of influenza A H1N1 upward reached its peak,influenza A H1N1 strain was the main virus.

关 键 词：流行性感冒 流感病毒 病原学监测 甲型H1N1 

分 类 号：R446.5[医药卫生—诊断学]